SAE0092
Mutanolisina
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)
Sinônimo(s):
Mutanolysin
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About This Item
Produtos recomendados
fonte biológica
Streptomyces sp. (Streptomyces globisporus ATCC 21553)
forma
lyophilized powder
atividade específica
≥4000 units/mg protein (biuret)
peso molecular
23 kDa
~18.5 kDa
Características
DNA free
concentração
≥40% protein
técnica(s)
nucleic acid detection: suitable
adequação
suitable for cell lysis
aplicação(ões)
life science and biopharma
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Aplicação
Proporciona lise celular branda para o isolamento de biomoléculas facilmente degradáveis e RNA de bactérias. Ele também foi usado na formação de esferoplastos para isolamento de DNA.
Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Ações bioquímicas/fisiológicas
A mutanolisina é uma N-acetilmuramidase. Como a lisozima, ela é uma enzima muralítica que cliva a ligação β-N-acetilmuramil-(1→4)-N-acetilglucosamina do polímero peptidoglicano-polissacarídeo da parede celular bacteriana. Suas porções terminais carboxi estão envolvidas no reconhecimento e na ligação a polímeros específicos da parede celular. A mutanolisina lisa Listeria e outras bactérias gram positivas, como Lactobacillus e Lactococcus.
Características e benefícios
Suitable for difficult-to-lyse samples.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Definição da unidade
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
forma física
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Informações legais
Ficoll is a registered trademark of Cytiva
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
Jennifer A Taylor et al.
Current protocols, 1(4), e80-e80 (2021-04-13)
The bacterial cell wall, composed of peptidoglycan (PG), provides structural integrity for the cell and is responsible for cell shape in most bacteria. Here we present tools to study the cell wall using a clickable PG-specific sugar, 2-alkyne muramic acid
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Artigos
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
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