SAE0091
Lisostafina
free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research
Sinônimo(s):
Endopeptidase glicil-glicina
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2 MG
R$ 1.459,00
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2 MG
R$ 1.459,00
About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.54
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Nome do produto
Lisostafina, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein
fonte biológica
microbial (Staphylococcus staphylotyticus)
Formulário
lyophilized powder
atividade específica
≥500 units/mg protein
peso molecular
27 kDa
Características
DNA free
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
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Aplicação
Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)
Ações bioquímicas/fisiológicas
A lisostafina é uma endopeptidase de zinco com um peso molecular de cerca de 25 kDa. Como a lisostafina cliva ligações cruzadas de poliglicina na camada de peptidoglicano da parede celular de espécies de Staphylococcus, ela provou ser útil para lise celular e também como possível medicamento antimicrobiano.
pH ideal para atividade: aprox. 7,5
pH ideal para atividade: aprox. 7,5
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.
Definição da unidade
One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.
forma física
Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Artigos
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
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