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D3937

Sigma-Aldrich

DirectLoad™ 1 kb DNA Ladder

ready-to-use marker for DNA electrophoresis

Sinônimo(s):

1 kb marker, 1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, 1kb marker for gel electrophoresis, DNA ladder, DNA marker, DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, ready to use DNA ladder, ready to use DNA marker

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About This Item

Código UNSPSC:
41105335
NACRES:
NA.25

Nível de qualidade

200

forma

liquid

uso

100 uses

adequação

suitable for electrophoresis (DNA)

temperatura de armazenamento

−20°C

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Descrição geral

Sigma′s DirectLoad™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.

Aplicação

Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.

Características e benefícios

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Componentes

Sigma′s DirectLoad™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Outras notas

Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.

Informações legais

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Methods in molecular biology (Clifton, N.J.), 597, 211-225 (2009-12-17)
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Artigos

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Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

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