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D5042

Sigma-Aldrich

123 bp DNA Ladder

for DNA electrophoresis

Sinônimo(s):

DNA Ladder for DNA electrophoresis, DNA marker

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About This Item

Código UNSPSC:
41105335
NACRES:
NA.25

Nível de qualidade

forma

liquid

uso

100 uses (0.1 mg size)
250 uses (250 μg size)

adequação

suitable for electrophoresis (DNA)

temperatura de armazenamento

−20°C

Aplicação

123 bp DNA Ladder has been used as a size marker in random amplified polymorphic DNA-polymerase chain reaction (PCR) analysis. It has also been used as a molecular marker in gel electrophoresis.

Componentes

Sigma′s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

Outras notas

Contains 35 fragments, 123 - 4182 bp
For optimal resolution, the recommended agarose gel concentration is 1.5%.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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Gene splicing and mutagenesis by PCR-driven overlap extension
Heckman KL and Pease LR
Nature Protocols, 2(4), 924-932 (2007)
Molecular characterization of lactic acid bacteria isolated from beef and stored using vacuum-packaging and advanced vacuum skin packaging systems
Inguane AS, et al.
CYTA-journal of Food (2011)
Straightforward procedure for laboratory production of DNA ladder
Lan VTT, et al.
Journal of Nucleic Acids, 2012 (2012)
Insulin-like growth factor I and II preserve myocardial structure in postinfarct swine
Kotlyar AA, et al.
British Heart Journal, 86(6), 693-700 (2001)
E J Gubbins et al.
The Journal of biological chemistry, 255(18), 8655-8662 (1980-09-25)
The organization and sequence of the rat preprolactin gene has been investigated. Analysis of two different plasmids containing pituitary cDNA inserts has provided the complete 681-nucleotide coding sequence of preprolactin as well as 17 nucleotides preceding the initiation codon and

Artigos

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Conteúdo relacionado

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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