S7025

50 bp DNA Step Ladder

for DNA electrophoresis

• Sinônimo(s): DNA Ladder, Step Ladder, 50 bp, 17 Fragments, 50-3000 bp
• Código UNSPSC: 41105335
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• forma: liquid
• uso: 100 uses
• adequação: suitable for electrophoresis (DNA)
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

Aplicação

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Componentes

Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

Outras notas

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable