Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Aplicação
Lambda DNA Mixed Digest has been used for DNA electrophoresis.[1]
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Componentes
Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Nota de preparo
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
Outras notas
For optimal resolution, the recommended agarose gel concentration is 0.4%.
A rapid (100 min) method for isolating high yield and quality DNA from leaves, roots and coleoptile of wheat (Triticum aestivum L.) suitable for apoptotic and other molecular studie
Hameed
International Journal of Agriculture and Biology, 383-387 (2004)
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
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