C8051
Colagenase
Sigma Blend Type H, ≥1.0 FALGPA units/mg solid
Sinônimo(s):
Clostridiopeptidase A
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About This Item
Produtos recomendados
![N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala](/deepweb/assets/sigmaaldrich/product/structures/805/876/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85/640/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85.png)
Nível de qualidade
forma
powder
caseinase activity
≥10 units/mg solid
atividade específica
≥1.0 FALGPA units/mg solid
peso molecular
68-130 kDa
temperatura de armazenamento
−20°C
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Aplicação
Collagenase from Clostridium histolyticum has been used:
- to harvest osteosarcoma 143B cells from collagen scaffold
- in tissue digestion of islets and carotid artery
- in the digestion of chorionic villi
- in enzymatic dissociation for ATP-based tumour chemosensitivity assay (ATP-TCA) in solid tumors
Ações bioquímicas/fisiológicas
Clostridium histolyticum produces two classes of collagenases. Clostridial collagenases are found to be highly effective than mammalian collagenases. It is capable of cleaving at multiple cleavage sites within the collagen triple helix. It is implicated in the bacterial invasion in gas gangrene.
Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL is used for digestion. The duration of reaction varyies from 15 minutes to several hours to yield a satisfactory efficiency of cell dissociation, without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preffered and Zn2+ is required for activity. This enzyme is tested for the release of hepatocytes at approximately 1 mg/mL conc., in a total volume of 100 mL for each rat liver.
A colagenase é ativada por quatro gramas de átomos de cálcio por mol de enzima. Ela é inibida por ácido etileno glicol-bis(beta-aminoetil éter)-N, N, N′,N′-tetracético, beta-mercaptoetanol, glutationa, ácido tioglicólico e 8-hidroxiquinolina.
Definição da unidade
Uma unidade de digestão de colágeno (CDU) libera peptídeos de colágeno do tendão de Aquiles bovino que equivalem em cor de ninidrina a 1,0 μmol de leucina em 5 horas em pH 7,4 a 37 °C na presença de íons de cálcio. Uma unidade de hidrólise de FALGPA hidrolisa 1,0 μmol de furilacriloil-Leu-Gli-Pro-Ala por min a 25 °C. Uma unidade de protease neutra hidrolisa caseína para produzir cor equivalente a 1,0 μmol de tirosina por 5 h em pH 7,5 a 37 °C. Uma unidade de clostripaína hidrolisa 1,0 μmol de BAEE por min em pH 7,6 a 25 °C na presença de DTT.
substrato
Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Protocolos
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
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