C8176

Colagenase

Sigma Blend Type L, ≤1.0 FALGPA units/mg solid

• Sinônimo(s): Clostridiopeptidase A
• Número CAS: 9001-12-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-582-9
• MDL number: MFCD00130830
• Número CE: 3.4.24.3 (BRENDA, IUBMB)
• Specific activity: ≤1.0 FALGPA units/mg solid

Propriedades

• Quality Segment: 200
• form: powder
• caseinase activity: ≥40 units/mg solid
• specific activity: ≤1.0 FALGPA units/mg solid
• mol wt: 68-130 kDa
• storage temp.: −20°C

Descrição

Application

Collagenase from Clostridium histolyticum, or Clostridiopeptidase A, has been used in a study to assess the synthesis and in vitro evaluation of macromolecular antitumour derivatives based on phenylenediamine mustard. Clostridiopeptidase A has also been used in a study to investigate the enzymatic debridement of burn wounds with collagenase.

Biochem/physiol Actions

A colagenase é ativada por quatro gramas de átomos de cálcio por mol de enzima. Ela é inibida por ácido etileno glicol-bis(beta-aminoetil éter)-N, N, N′,N′-tetracético, beta-mercaptoetanol, glutationa, ácido tioglicólico e 8-hidroxiquinolina.

Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.

Other Notes

Uma unidade de digestão de colágeno (CDU) libera peptídeos de colágeno do tendão de Aquiles bovino que equivalem em cor de ninidrina a 1,0 μmol de leucina em 5 horas em pH 7,4 a 37 °C na presença de íons de cálcio. Uma unidade de hidrólise de FALGPA hidrolisa 1,0 μmol de furilacriloil-Leu-Gli-Pro-Ala por min a 25 °C. Uma unidade de protease neutra hidrolisa caseína para produzir cor equivalente a 1,0 μmol de tirosina por 5 h em pH 7,5 a 37 °C. Uma unidade de clostripaína hidrolisa 1,0 μmol de BAEE por min em pH 7,6 a 25 °C na presença de DTT.

Informações de segurança

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Classe de armazenamento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves