Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

C6079

Sigma-Aldrich

Collagenase + protease inhibitor

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

Sinônimo(s):

Collagenase preparation, Protease inhibitor solution

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número da licença da enzima:
3.4.24.3 (BRENDA, IUBMB)
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

bacterial (Clostridium histolyticum)

Nível de qualidade

200

forma

solid

atividade específica

≥800 CDU/mg solid
2-5 FALGPA units/mg solid

peso molecular

68-130 kDa

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Informações sobre genes

rat ... LOC296948(296948)

Categorias relacionadas

Aplicação

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.

Ações bioquímicas/fisiológicas

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca2+) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn2+ is required for activity. This product is used if the collagenase does not require a significant protease activity.

Outras notas

Selected lots of collagenase Type XI blended with protease inhibitor to limit the tryptic enzyme activities in pancreatic tissue. The formulation was developed through collaboration with several outside laboratories.

Qualidade

Also contains clostripain and non-specific neutral protease activities.

inibidor

Nº do produto
Descrição
Preços

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 5

1 of 5

Colagenase D from Clostridium histolyticum

Roche

COLLD-RO

Colagenase D

Colagenase Sigma Blend Type H, ≥1.0 FALGPA units/mg solid

Sigma-Aldrich

C8051

Colagenase

Colagenase 0.2 μm filtered, suitable for release of physiologically active rat epididymal adipocytes, Type II-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C1764

Colagenase

Sigma-Aldrich

Sigma-Aldrich

M9445

Matrix Metalloproteinase-2 from mouse

Colagenase P from Clostridium histolyticum

Roche

COLLP-RO

Colagenase P

Association of immune system gene polymorphisms with quantitative features which are pathogenetically important in chronic viral hepatitis.
Goncharova, I., et al.
Molecular Biology, 42, 242-246 (2008)
Ashutosh Jamloki et al.
Bioorganic & medicinal chemistry letters, 16(14), 3847-3854 (2006-05-10)
A quantitative structure-activity relationship (QSAR) study has been performed on 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases (MMPs) and a bacterial collagenase known as Clostridium histolyticum collagenase (ChC) to understand the structural features influencing the affinity of these inhibitors towards the
Austin P Huffman et al.
JCI insight, 5(10) (2020-04-24)
The role CD4+ T cells play in tumor immunity is less well appreciated than the cytotoxic role of CD8+ T cells. Despite clear evidence for CD4+ T cell dependency across multiple immunotherapies, the mechanisms by which CD4+ T cells infiltrate
C J Swanson et al.
Human immunology, 62(7), 739-749 (2001-06-26)
Recent progress in human islet transplantation demonstrates the feasibility of using purified human islets for treatment of type 1 diabetes mellitus; however, a shortage of human pancreata remains a major obstacle. This report describes methods to isolate porcine islets using
Brandon M Blobner et al.
American journal of physiology. Gastrointestinal and liver physiology, 321(5), G449-G460 (2021-09-16)
Chronic pancreatitis (CP) is a complex inflammatory disorder with numerous associated genetic and environmental risk factors. The most distressing characteristic of CP is recalcitrant pain, often requiring surgical resection including total pancreatectomy with islet autotransplantation (TPIAT). We studied five consented
Exibir todos os documentos relacionados

Protocolos

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica