Pular para o conteúdo
Merck
Todas as fotos(5)

Documentos Principais

270733

Sigma-Aldrich

Água

Water
1 of 1 reviewers received a sample product or took part in a promotion

suitable for HPLC

Sinônimo(s):

Aqua, Óxido de di-hidrogênio

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Fórmula linear:
H2O
Número CAS:
Peso molecular:
18.02
Beilstein:
2050024
Número CE:
Número MDL:
Código UNSPSC:
12190000
ID de substância PubChem:
NACRES:
NA.21
Preço e disponibilidade não estão disponíveis no momento.

grau

HPLC grade

Nível de qualidade

densidade de vapor

<1 (vs air)

pressão de vapor

3 mmHg

Formulário

liquid

técnica(s)

HPLC: suitable

resíduo de evaporação

<0.0003%

índice de refração

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

p.e.

100 °C (lit.)

pf

0 °C (lit.)

densidade

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

Absorção UV

λ: 205 nm Amax: 0.01
λ: 210 nm Amax: 0.01
λ: 250-400 nm Amax: 0.005

cadeia de caracteres SMILES

O

InChI

1S/H2O/h1H2

chave InChI

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

A água adequada para HPLC é comumente usada como um solvente para eluição isocrática tanto na cromatografia de cátions quanto de ânions. Além disso, é usada como um componente para preparar soluções-estoque padrão e para evaporação sob um fluxo de nitrogênio em banho-maria para concentrar a amostra antes da análise LC-MS-MS.[1] [2]

Aplicação

A água é usada como:
  • Solvente para preparar tampões durante o processo de lise celular.[3]
  • Componente da fase móvel durante a detecção de matriz de diodos HPLC–UV–para a separação e a detecção dos analitos.[4]

Embalagem

M-Bottle for Solvents
As a global leader in lab reagents, we are constantly looking for new ways to optimize the safety of our products. The newly developed 4L solvent bottle design features advanced sealing technology that eliminates leaks to make the handling of solvents safer and more convenient than ever before.
See all the new features here!

Nota de preparo

Produto filtrado em filtro de 0,2 μm

Outras notas

Aviso importante
O artigo número 270733-6X1L será descontinuado. Encomende o frasco único 270733-1L, que é fisicamente idêntico, com exatamente as mesmas especificações.

Produtos recomendados

Descubra os reagentes LiChropur ideais para análise por HPLC ou LC-MS

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number

Não está vendo a versão correta?

Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 3

1 of 3

Água for ultratrace analysis, ACS reagent

Supelco

14211

Água

Acetonitrilo HPLC Plus, &#8805;99.9%

Sigma-Aldrich

34998

Acetonitrilo

Água ACS reagent

Sigma-Aldrich

320072

Água

Identification of the cysteine protease legumain as a potential chronic hypoxia-specific multiple myeloma target gene
Ada-Sophia C et al.
Cells, 11, 292-292 (2022)
Rapid confirmatory assay for the simultaneous detection of ronidazole, metronidazole and dimetridazole in eggs using liquid chromatography-tandem mass spectrometry
Els D et al.
Analyst, 125, 1533-1535 (2000)
Milk ultrafiltrate analysis by ion chromatography and calcium activity for SMUF preparation for different scientific purposes and prediction of its supersaturation
Joseph D et al.
International dairy journal, 68, 60-69 (2017)
Optimization and validation of HPLC-UV-DAD and HPLC-APCI-MS methodologies for the determination of selected PAHs in water samples
Titato, Guilherme T M et al.
Journal of Chromatographic Science, 44, 35-40 (2006)
Manolis Mandalakis et al.
Journal of chromatography. A, 1217(1), 143-150 (2009-12-08)
The analysis of amino acids by gas chromatography mass spectrometry (GC-MS) after their derivatization with N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide was investigated as an alternative approach for the determination of free (FAA) and combined amino acids (CAA) in aerosols. This technique showed excellent linearity

Artigos

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Ultrapure water from Milli-Q® system shown free of BPA, suitable for embryology research, minimizing endocrine disruptor interference.

A água ultrapura do sistema Milli-Q® mostrou-se livre de BPA e adequada para pesquisas embriológicas, minimizando a interferência de desreguladores endócrinos.

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

Ver tudo

Protocolos

A guide to create solvent systems used for the thin-layer chromatography assay of Novabiochem products.

Questions

1–8 of 8 Questions  
  1. Is this water suitable for lal test method ? Is pyrogen free ? Is there a specification?

    1 answer
    1. This product is not suitable for the Limulus Amebocyte Lysate test method (LAL). Specifications for this product are available in the documents section of the webpage, but pyrogen levels are not specified. This product is not certified as pyrogen-free and is not sterile. This product is intended for use as a solvent for High-Performance Liquid Chromatography mobile phases.

      Helpful?

  2. Will dispensing small quantities and resealing the container lead to air dissolving in the water? I intend to use 50ml-100ml for my experiments.

    1 answer
    1. Opening a container that is not isolated from the atmosphere could results in ambient air dissolving in the water remaining inside the container. The rate of of air introduction would need to be monitored for a given set of conditions such as temperature, air exchange in the laboratory, and relative humidity.

      Helpful?

  3. Do you know the resistivity value @ 25°C?

    1 answer
    1. The resistivity of this product has not been determined.

      Helpful?

  4. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  5. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  6. How is this water purified? is it distilled?

    1 answer
    1. The water purification process involves several steps:
      • The water initially passes through a carbon exchange tank to remove organic compounds.
      • It is then filtered using a 1μm PE cartridge filter to remove any remaining impurities.
      • Inorganic compounds are eliminated through deionization tanks, ensuring high purity. The resistivity of the water is checked during this stage.
      •The water undergoes sterilization using UV light to eliminate any microorganisms.
      • Finally, the water is filtered through a 0.2μm cartridge filter, and the resulting HPLC grade water is packaged in glass bottles.
      Note: This information provides an overview of the typical water purification process and may vary depending on specific applications or systems.

      Helpful?

  7. Do you know the concentration of TOC in this water?

    1 answer
    1. The concentration of TOC in this product is not determined. Product 78533, Water for TOC Analysis, may be of interest.
      Please see the link below to review this product option:
      https://www.sigmaaldrich.com/product/sial/78533

      Helpful?

  8. Is this product (HPLC grade water #270733) DNase-, RNase- and protease-free?

    1 answer
    1. The item is not tested for the presence of DNA, RNA, and protease.

      Please see the links below to review product options that include this testing:
      W4502
      https://www.sigmaaldrich.com/US/en/product/sigma/w4502
      95284
      https://www.sigmaaldrich.com/US/en/product/sigma/95284

      Note that the above options are NOT tested for suitability in HPLC.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

    • Reviews 3
    • Votes 9
    5 out of 5 stars.

    Good, pure water

    Water was high purity, HPLC grade. We didn't actually use it for HPLC but it seemed very clean and pure for our purposes.

    Helpful?

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica