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T9284

Sigma-Aldrich

Triton X-100

BioXtra

Sinônimo(s):

t-Octilfenoxipolietoxietanol, Polietilenoglicol terc-octilfenil éter

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About This Item

Fórmula linear:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Número CAS:
Beilstein:
2315025
Número MDL:
Código UNSPSC:
12161900
ID de substância PubChem:
NACRES:
NA.21

fonte biológica

synthetic

Nível de qualidade

descrição

non-ionic

linha de produto

BioXtra

forma

liquid

peso molecular

micellar avg mol wt 80,000
average mol wt 625

número de agregação

100-155

técnica(s)

permeability assay: suitable

Impurezas

≤0.05% Phosphorus (P)

resíduo de ignição

≤0.4%

CMC

0.2-0.9 mM (20-25°C)

temperatura de transição

cloud point 65 °C
pour point ~7 °C

solubilidade

water: soluble

densidade

1.065 g/cm3 at 20 °C (68 °F)

traços de ânion

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

traços de cátion

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.1%
Pb: ≤0.001%
Zn: ≤0.0005%

HLB

13.5

cadeia de caracteres SMILES

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

chave InChI

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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Descrição geral

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Aplicação

Triton X-100 has been used:
  • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
  • In the blocking solution during immunocytochemistry procedure
  • During deglycosylation procedure
  • For membrane solubilization
  • For cell permeabilization
  • In decellularization of porcine heart valve tissue

Ações bioquímicas/fisiológicas

Tensoativo não iônico amplamente utilizado para recuperar componentes de membrana em condições brandas não desnaturantes.

Características e benefícios

  • BioXtra Grade product for your Cell Biology and Biochemical research
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

483.8 °F - closed cup

Ponto de fulgor (°C)

251 °C - closed cup

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Membrane and acto-myosin tension promote clustering of adhesion proteins.
Delanoe-Ayari H, et al.
Proceedings of the National Academy of Sciences of the USA, 101(8), 2229-2234 (2004)
Structural, metabolic and developmental evaluation of ovulated rabbit oocytes before and after cryopreservation by vitrification and slow freezing.
Theriogenology, 74(5), 847-855 (2010)
Detection of fetal DNA in maternal plasma by microarray coupled with emulsions PCR.
Ge Q, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 369(1), 82-88 (2006)
Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats.
Kwon TH et al.
American Journal of Physiology: Renal Physiology, 288, F673-F673 (2005)
Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea.
Hong JW et al.
Investigative Ophthalmology & Visual Science, 42, 2795-2795 (2001)

Protocolos

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

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