To avoid contamination, sterile filtration is highly recommended prior to cell culture application. Upon verification, the lab has not formulated a protocol for cell culture use. However, the following citation on M1753 for cell culture application may be of interest as a protocol reference: Im, H. (2011). "Mouse Embryonic Stem Cell Maintenance for Differentiation." Bio-101: e145. DOI: 10.21769/BioProtoc.145.
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M1753
1-Thioglycerin
≥97%
Synonym(e):
α-Monothioglycerol, α-Thioglycerol, 3-Mercapto-1,2-propandiol
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100 ML
€ 200,00
500 ML
€ 789,00
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100 ML
€ 200,00
500 ML
€ 789,00
About This Item
Lineare Formel:
HSCH2CH(OH)CH2OH
CAS-Nummer:
Molekulargewicht:
108.16
Beilstein:
1732046
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352201
PubChem Substanz-ID:
NACRES:
NA.25
Empfohlene Produkte
Assay
≥97%
Form
liquid
Brechungsindex
n20/D 1.527 (lit.)
bp
118 °C/5 mmHg (lit.)
Dichte
1.25 g/mL at 25 °C (lit.)
Lagertemp.
2-8°C
SMILES String
OCC(O)CS
InChI
1S/C3H8O2S/c4-1-3(5)2-6/h3-6H,1-2H2
InChIKey
PJUIMOJAAPLTRJ-UHFFFAOYSA-N
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Allgemeine Beschreibung
1-Thioglycerin stimuliert die Synthese von Porphyrin in aerob wachsenden Escherichia coli. [1] Es ist ein Inhibitor der Glycerinkinaseaktivität in vitro und in situ.
Anwendung
Angaben zur Herstellung
Dieses Produkt ist mit Ethanol mischbar (1 ml/ml, 50 %, v/v) und erzeugt eine klare, farblose Lösung. Es ist auch mit Wasser mischbar (0,1 M).
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flammpunkt (°F)
210.2 °F - closed cup
Flammpunkt (°C)
99 °C - closed cup
Persönliche Schutzausrüstung
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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1-Thioglycerol (TG) stimulates the synthesis of porphyrin in aerobically growing Escherichia coli. Here the levels of delta-aminolevulinate biosynthetic enzymes in untreated and TG-treated E. coli THU and PUC2 (a mutant of THU which overproduces porphyrins in the presence of thiols)
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What could be causing contamination of cell culture experiments while using M1753? Also, how should M1753 be added to the cell culture media?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the concentration of the 1-thioglycerol?
1 answer-
The liquid is not a solution of 1-thioglycerol in some solvent. Rather, it is a neat liquid (i.e., essentially pure 1-thioglycerol). Based on a density of 1.245 g/mL and a molecular weight of 108.2 g/mole, one can calculate an effective molarity of 11.5 M. This information may be found on the M1753 product information sheet.
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