Stock solutions may be refrigerated for up to 2 weeks. For long term storage, the stock solution may be stored at -20°C in aliquots for 6 - 12 months. Avoid repeated freeze/thaw cycles. This product is not sterile. Stock solutions should be filter sterilized using a 0.22 micron filter.
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H0396
Hydrokortison - wasserlöslich
Hydrocortisone-Water Soluble
1 of 2 reviewers received a sample product or took part in a promotion
BioReagent, suitable for cell culture
Synonym(e):
Cyclodextrin-eingekapseltes Hydrokortison
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100 MG
€ 211,00
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100 MG
€ 211,00
About This Item
Biologische Quelle:
plant (wild yam)
Form:
crystalline
Empfohlene Produkte
Biologische Quelle
plant (wild yam)
Qualitätsniveau
Produktlinie
BioReagent
Form
crystalline
Methode(n)
cell culture | mammalian: suitable
Löslichkeit
H2O: 400 mg/mL
Versandbedingung
ambient
Lagertemp.
room temp
SMILES String
C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
InChIKey
JYGXADMDTFJGBT-VWUMJDOOSA-N
Angaben zum Gen
human ... ABCB1(5243) , CYP3A4(1576) , NR3C1(2908) , SERPINA6(866)
mouse ... Abcb1a(18671) , Abcb1b(18669) , Ifng(15978) , Nos2(18126)
rat ... Ar(24208) , Nr3c1(24413)
Allgemeine Beschreibung
Hydrocortison hat das Potenzial, die Bildung von Blut-Hirn-Schranke-Eigenschaften in serumfreien Nährmedien einzuleiten. Es wird für die Anwendung in epithelialen und endothelialen adhärenten Zellkulturen empfohlen.
Anwendung
Wasserlösliches Hydrocortison wird als Zusatz zu Dulbeccos Modified Eagle Medium (DMEM) / Medium mit hohem Glukosegehalt zum Kultivieren von Brustalveolarzellen (MAC-T-Zellen) eingesetzt. Es wurde auch zum Untersuchen seiner Wirkung auf die Kationenabsorption von Bogengangepithel durch den epithelialen Natriumkanal eingesetzt.
Verpackung
Packungsgrößen basieren auf Hydrocortison
Ähnliches Produkt
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Repr. 1A
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Gloves
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Dopo avre sciolto in acqua Hydrocortisone (H0396) a che temperatura deve essere conservato?
1 answer-
Helpful?
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Which temperature would you suggest to store "400 mg/mL stock solution"? and for how long? Do you suggest to prepare H0396 in a sterile environment and further filter working solution using the 0.22um filter before cell culture use?
1 answer-
Stock solutions may be refrigerated for up to 2 weeks. For long term storage we recommend -20 deg C in aliquots for 6 - 12 months. Avoid repleated freeze/thaw cycles. This product is not sterile. Stock solutions should be filter sterilized using a 0.22 micron filter.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How do I calculate the molarity of product H0396, Hydrocortisone-Water Soluble?
1 answer-
Product H0396 (Hydrocortisone-Water Soluble) is a complex of Hydrocortisone and cyclodextrin. Cyclodextrin is used to aid in the solubility of Hydrocortisone into water. In order to prepare a molar solution of Hydrocortisone you will have to base it on the Hydrocortisone content in the solution and not the entire complex. Product H0396 is formulated to contain approximately 85 mg Hydrocortisone per gram of powder. Based on this average, if you are preparing a 400 mg/ml stock solution, there will be approximately 34 mg of Hydrocortisone in 1 ml. Below is the calculated molarity of Hydrocortisone when the complex is dissolved at 400 mg/ml in water. molecular weight X unknown molarity X volume = complex weight X complex ratio362.46 g/mol X Molarity X 1 ml = 400 mg X 0.085 Molarity = 0.094 MHydrocortisone molecular weight = 362.46 g/molmolarity (unknown) = mol/Lvolume = 1mlcomplex weight = 400 mgcomplex ratio = 85 mg Hydrocortisone/1 gm complex
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