Yes, it is light sensitive and should be protected from prolonged exposure to light.
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H0888
Hydrocortison
BioReagent, suitable for cell culture
Hydrocortisone
Synonym(e):
11β,17α,21-Trihydroxypregn-4-en-3,20-dion, 17-Hydroxykortikosteron, 4-Pregnen-11β,17α,21-triol-3,20-dion, Cortisol, Kendall’s Compound F, Reichstein’s Substance M
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 g | Warenkorb auf Verfügbarkeit prüfen | € 57,00 |
5 g | Warenkorb auf Verfügbarkeit prüfen | € 220,00 |
10 g | Warenkorb auf Verfügbarkeit prüfen | € 314,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C21H30O5
CAS-Nummer:
Molekulargewicht:
362.46
UNSPSC Code:
12352209
NACRES:
NA.77
PubChem Substance ID:
EC Number:
200-020-1
Beilstein/REAXYS Number:
1354819
MDL number:
Biological source:
soybean
Form:
powder
Assay:
≥98%
Technischer Dienst
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Unterstützung erhaltenbiological source
soybean
Quality Segment
product line
BioReagent
assay
≥98%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
211-214 °C (lit.)
solubility
chloroform/ethanol (1:1): 50 mg/mL, clear, colorless to very faintly yellow
shipped in
ambient
storage temp.
room temp
SMILES string
[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
InChI key
JYGXADMDTFJGBT-VWUMJDOOSA-N
Gene Information
human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)
General description
Application
Biochem/physiol Actions
Primäres Glucocorticoid im Sekret der Nebennierenrinde
Primäres Glucocorticoid im Sekret der Nebennierenrinde. Hat ein dreimal so hohes entzündungshemmendes Potential wie Corticosteron bei einem deutlich geringeren Potential zur Na2+-Retention.
Hydrocortison (auch Cortisol genannt) ist ein endogenes Glucokortikoid, das von der Nebenniere abgesondert wird. Es wird von der Hypothalamus-Hypophysen-Nebennierenrinden(HPA)-Achse produziert. Hydrocortison interagiert mit Glucokortikoidrezeptoren und reguliert verschiedene Signalwege. Es wird mit der Herbeiführung der Energiemobilisierung, der Erhöhung der Hirndurchblutungsgeschwindigkeit und des lokalen Glukoseverbrauchs assoziiert. Es steht auch mit einer Erhöhung der kardiovaskulären Leistung und Atmung, der Umverteilung von Blut, der Lieferung von Molekülen und Energie an das Gehirn und in die Muskeln sowie der Modulation der Immunreaktion in Verbindung.
Physical form
Pulver-RT; Stammlösung in Arbeitsaliquoten eingefroren, wiederholtes Einfrieren/Auftauen ist zu vermeiden
Preparation Note
Für die Herstellung von 50 μg/ml Stammlösung 1,0 ml reines Ethanol zu 1,0 mg Produkt geben, zum Auflösen sanft schwenken, 19 ml steriles Medium hinzugeben.
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Dieser Artikel | |||
|---|---|---|---|
| description BioReagent, suitable for cell culture | description γ-irradiated, powder, BioXtra, suitable for cell culture | description 50 μM, sterile-filtered, BioXtra, suitable for cell culture | description meets USP testing specifications |
| biological source soybean | biological source synthetic (organic) | biological source non-animal source | biological source plant (wild yam) |
| assay ≥98% | assay ≥80% (HPLC) | assay - | assay 97.0-102.0% dry basis |
| Quality Level 200 | Quality Level 200 | Quality Level 500 | Quality Level 300 |
| product line BioReagent | product line BioXtra | product line BioXtra | product line - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| form powder | form powder | form liquid | form powder |
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signalword
Danger
hcodes
Hazard Classifications
Repr. 1A - STOT RE 2
target_organs
Adrenal gland,Bone marrow
Lagerklasse
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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Is it light sensitive?
1 answer-
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How long can the powder be stored at RT? Reconstituting 1000mg to obtain 50mg/mL calls for 20mL of chloroform/ethanol, I need 100 aliquots of 50mg/ul in 10uL, therefore 20mL is too much, can I use only 50mg and save the rest for later use?
1 answer-
This product is considered stable but may degrade over time. A periodic retest date is assigned to confirm quality, and the lot-specific retest date is reported on the Certificate of Analysis. It is acceptable to weigh out only the required amount of hydrocortisone powder (e.g., 50 mg), dissolve it in the appropriate volume of solvent, and save the remaining powder for later use. The remaining powder should be stored tightly closed, protected from moisture and light, and at room temperature.
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How long can you keep the powder stored at 4°C without a negative impact on effectiveness?
1 answer-
This product is typically stored at room temperature. Stability studies at 4°C have not been conducted. However, storing the material at refrigerated temperatures is not expected to adversely effect the activity. Upon removal from the refrigerator, the bottle should be allowed to equilibrate prior to opening to avoid condensation and moisture uptake.
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How long is the stock solution reconstituted in absolute ethanol stable for at room temperature and 4C?
1 answer-
Stock solutions are most stable when stored in aliquots at -20°C, protected from light. Solutions are expected to remain stable for 2 - 4 weeks when refrigerated at 2-8°C. The stability of this product in solution at room temperature has not been evaluated. Commercially available products, such as Solu Cortef® solutions, stored at room temperature at >2 mg/mL, are recommended to be discarded after 3 days or sooner if a change in color or turbidity is noted.
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Can this compound be dissolved in DMSO, aliquoted to avoid the freeze thaw cycle, and store at -20C instead of chloroform:ethanol mixture?
1 answer-
The solubility of this product in DMSO has not been determined. However, various sources state that this product is soluble in DMSO at a minimum of 20 mg/mL.
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How long can the powder be stored after date of receipt?
1 answer-
This product, while considered stable, may be subject to degradation over time and is assigned a periodic retest date to confirm quality. The lot specific retest date is reported on the Certificate of Analysis. Please see the link below or navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/h0888#product-documentationHelpful?
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How stable is hydrocortisone? I use it and supplement a medium but I administer it every 48 hours, can I have my stock for 7 days and it does not lose its effect or do I have to defrost it every time I use it so that it does not lose its effect?
1 answer-
The stability of this product in solution has not been determined. To assure optimum performance, it is recommended that solutions be stored in frozen aliquots at -20°C. However, the water soluble version of Hydrocortisone, available as product H0396, is estimated to be stable for 1 - 2 weeks when refrigerated at 2 - 8°C. Note that a publication, J. AM. PHARM. ASSOC., 49, 253 (1960), determined that solutions prepared in ethanol at 40mg/mL and stored at 25°C decline by 50% in potency after 160 days.
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Can it be dissolved completely in absolute ethanol, and stored per instructions (instead of 1:1 chloroform and ethanol)?
1 answer-
A stock solution can be prepared by reconstituting the product at 1 mg/ml in 100% EtOH. Gently swirl to dissolve. Then dilute to 50 ug/ml with sterile culture media, aliquot, and store at -20 °C. As a frozen aliquot, this should be stable for at least 3-4 months. Avoid the freeze/thaw cycle.
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