Unfortunately, no data for Product D4008's use with flow cytometry application is determined. However, several academic publications associated with this product's use in flow cytometry experiments may provide details that can assist with the experimental design. Please see the link to those publications below:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C26&q=%22Dansylcadaverine%22+%22D4008%22+%22flow+cytometry%22&btnG=
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D4008
Dansylcadaverin
≥97% (TLC)
Synonym(e):
N-(5-Aminopentyl)-5-dimethylamino-1-naphthalinsulfonamid
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250 MG
€ 74,60
100 MG
€ 99,90
1 G
€ 601,00
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Größe auswählen
Ansicht ändern
250 MG
€ 74,60
100 MG
€ 99,90
1 G
€ 601,00
About This Item
Empirische Formel (Hill-System):
C17H25N3O2S
CAS-Nummer:
Molekulargewicht:
335.46
Beilstein:
2951070
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352108
PubChem Substanz-ID:
NACRES:
NA.32
Empfohlene Produkte
Qualitätsniveau
Assay
≥97% (TLC)
Form
powder
Lagertemp.
−20°C
SMILES String
CN(C)c1cccc2c(cccc12)S(=O)(=O)NCCCCCN
InChI
1S/C17H25N3O2S/c1-20(2)16-10-6-9-15-14(16)8-7-11-17(15)23(21,22)19-13-5-3-4-12-18/h6-11,19H,3-5,12-13,18H2,1-2H3
InChIKey
MLEBFEHOJICQQS-UHFFFAOYSA-N
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Allgemeine Beschreibung
Dansylcadaverine, also called as monodansylcadaverine (MDC), is a lysosomotropic agent. It is present in acidic and lipid rich regions. Dansylcadaverine also acts as a solvent polarity probe.[1] It inhibits vesicular transport and decreases the rotavirus sequestering in L cells, when pre-treated.[2]
Anwendung
Dansylcadaverine in methanol has a reported λex of 335 nm and λem of 512 nm. It has been used:
- as an endocytosis inhibitor in human proximal tubular HK-2 cells,(62) mouse J774A.1 macrophages and human A549 epithelial cells(63)
- to label autophagic vacuoles in cardiomyocytes for fluorescence microscopy studies(64)
- in monodansylcadaverine (MDC) staining of fibroblasts in Ankylosing spondylitis (AS) samples(65)
Biochem./physiol. Wirkung
Der autofluoreszierende Farbstoff Dansylcadaverin, oder Monodansylcadaverin (MDC), wird häufig verwendet, um Autophagie zu überwachen. MDC wird, durch eine Kombination von Ionenfallen und spezifische Wechselwirkungen mit Membranlipiden, bevorzugt in autophagischen Vakuolen anreichert.
Verpackung
Bottomless glass bottle. Contents are inside inserted fused cone.
air sensitive, handled under inert gas
Sonstige Hinweise
Die Löslichkeit von Dansylcadaverin wird in Methanol getestet (10 mg/mL). Es ist bekannt, dass Dansylcadaverin auch in DMSO und Essigsäure löslich ist.
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Gloves
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Fluorescence properties and staining behavior of monodansylpentane, a structural homologue of the lysosomotropic agent monodansylcadaverine
Niemann A, et al.
The Journal of Histochemistry and Cytochemistry, 49(2), 177-185 (2001)
Dansylcadaverine and cytochalasin D enhance rotavirus infection of murine L cells
Bass DM, et al.
Virology, 212(2), 429-437 (1995)
Raúl A Almeida et al.
Veterinary research communications, 35(2), 89-101 (2011-01-06)
Research on mastitis in dairy cows caused by Escherichia coli has reported the emergence of strains capable of inducing chronic mastitis and that these strains adhered to and internalized into bovine mammary epithelial cells better than strains of E. coli
Melissa S Hillwig et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(3), 1093-1098 (2011-01-05)
RNase T2 enzymes are conserved in most eukaryotic genomes, and expression patterns and phylogenetic analyses suggest that they may carry out an important housekeeping role. However, the nature of this role has been elusive. Here we show that RNS2, an
Shih T Khew et al.
Biomaterials, 31(16), 4600-4608 (2010-03-13)
Transglutaminase (TGase)-modified proteins are commonly observed in a wide range of biological systems. Therefore, the identification of TGase substrates and respective consensus sites may contribute to a better understanding of the physiological role of TGase. In this study, we identified
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Can I use Dansylcadaverine D4008 for detection of autophagy using flow cytometry? if Yes which laser should we use to detect the MDC signal? Di you have any recommendations regarding the final concentration of MDC for detection of autophagy in lymphocyte?
1 answer-
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Can Dansylcadaverine be use as a marker of autophagic vacuoles?
1 answer-
Dansylcadaverine is a lysosomotropic compound that can be used for the identification of autophagic vacuoles. Please refer to the literature for application-specific protocols.
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What is Product D4008, Dansylcadaverine, soluble in?
1 answer-
We test the solubility of this product in acetic acid at a concentration of 50 mg/mL, with the application of heat and sonication. It has also been reported as being soluble in methanol and DMSO. We have not validated this claim in our labs.
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What is the solution stability of Product D4008, Dansylcadaverine?
1 answer-
According to one source a stock solution of monodansylcadaverine was prepared in methanol at 50 mM and stored frozen at -20°C. No time frame for long-term solution stability was given.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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