11699105001

PCR Buffer Without MgCl₂, 10x concentrated

solution, pkg of 3 × 1 mL

• Synonym(e): buffer
• UNSPSC-Code: 41105600

Eigenschaften

• Form: solution
• Qualitätsniveau: 100
• Verpackung: pkg of 3 × 1 mL
• Hersteller/Markenname: Roche
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

Anwendung

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).[1][2]

Physikalische Form

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Sonstige Hinweise

For general laboratory use.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash