DNA-Verunreinigungen können durch verschiedene Reagenzien in die PCR gelangen. Um das Risiko von DNA-Verunreinigungen bei der PCR zu minimieren, bieten wir 10X MTP Taq-Puffer zur Verwendung in Kombination mit der MTP Taq-DNA-Polymerase (Produktnr. D7442) an. Jede Charge des MTP-Taq-Puffers wird strengen Qualitätskontrolltests unterzogen, um das Nichtvorhandensein von DNA-Verunreinigungen sicherzustellen. Es sollten nur DNA-freie Reagenzien für PCR-Reaktionen mit MTP Taq-DNA-Polymerase verwendet werden, um falsch-positive PCR-Resultate zu vermeiden.
Anwendung
10X MTP™-Taq-Puffer wird wie folgt verwendet:
zur Amplifikation von bakteriellen 16S-rRNA-Genen aus aufgereinigter DNA[1]
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
In order to understand the effect of human practices on microbial mats organisation, the study aimed to investigate the biodiversity within microbial mats from exploited and abandoned salterns. Despite several attempts, archaeal 16S rRNA gene fragment sequences were not obtained
Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology.
B Cherie Millar et al.
Journal of clinical microbiology, 40(5), 1575-1580 (2002-05-01)
Increased visceral adipose tissue and dysbiosis in the overweight and obese promote chronic inflammation. The aim of this study was to compare the effects of moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) on the gut-adipose tissue cross-talk in
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
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