HC08

Fluorescent Mounting Media, Aqueous

This Fluorescent Mounting Media, Aqueous is validated for use in Immunofluorescence for the detection of Fluorescent Mounting Media Aqueous.

• UNSPSC-Code: 41116133
• NACRES: NA.47

Eigenschaften

• Form: liquid
• Enthält nicht: preservative
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

An aqueous mounting medium designed to preserve and enhance the labeling of immunoreactivity in tissue sections. Sustains the fluorescent signal from labeled samples on slides. Inclusion of 4,6-diamidino-2-phenylindole (DAPI; Cat. No. 268298) allows for the visualization of unlabeled (non-apoptotic) cells. Suitable for use with a wide variety of fluorophores.

Aqueous mounting media designed to preserve and enhance the labeling of tissue sections or cell preparations fixed on slides. Suitable for many fluorophores including fluorescein, Oregon Green, Alexa 488, rhodamine, Texas Red, Cyanine (Cy) 3, and Alexa 546. Inclusion of DAPI in the Fluorescent Mounting Media allows for the visualization of all cells at an excitation wavelength of 330-380 nm.

Anwendung

Immunofluorescence (see comments)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

8 mL in glycerol solution containing 25 mg/ml 1,4-DiAzaBicyclo-[2.2.2]Octane (DABCO) in Tris buffer and 0.1 µg/ml 4,6-DiAmidino-2-PhenylIndole (DAPI).

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Sonstige Hinweise

For best results: following the final aqueous step of the staining procedure remove excess fluid around the specimen, apply 1-2 drops of Fluorescent Mounting Media to the specimen and carefully place a coverglass on top. Seal the border of the coverglass with nail polish. Store mounted specimens at 4°C in the dark.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1