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The formulation of this aqueous mounting medium is considered proprietary.
Fortfahren mit
F6182
Fluoroshield™
liquid
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
20 mL | Warenkorb auf Verfügbarkeit prüfen | € 94,30 |
Über diesen Artikel
UNSPSC Code:
12171500
NACRES:
NA.47
Technischer Dienst
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Unterstützung erhaltenProduktname
Fluoroshield™, histology mounting medium
Quality Segment
form
liquid
technique(s)
immunofluorescence: suitable
pH range
7.8-8.2
solubility
water: soluble
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
General description
Fluoroshield ist ein wässriges Fixiermittel zur Erhaltung der Fluoreszenz von Gewebe und Zellabstrichen. Diese besondere Formel verlängert nicht nur die Fluoreszenz, sondern verhindert auch ein schnelles Photobleaching (Verblassen) von Fluorochromen wie FITC, Texasrot®, AMCA, Cy®, Cy3™, Cy5, Alexa Fluor™ 488, Alexa Fluor™ 594, grün fluoreszierendem Protein (GFP), Tetramethylrhodamin, R-Phycoerythrin (R-PE), Phycocyanin (PC) und Allophycocyanin (APC).
Application
Fluoroshield™ wird wie folgt verwendet:
- als Fixiermittel für kultivierte Megakaryozyten und immungefärbte Thrombozyten bei konfokalen mikroskopischen Untersuchungen[1]
- als Fixiermittel für 4′,6-Diamidino-2-phenylindol(DAPI)-gefärbte Herzgewebeschnitte bei Immunfluoreszenzstudien[2]
- zur Erhaltung der Fluoreszenz von C6-Zellabstrichen
Other Notes
Fluoroshield ist ein Fixiermittel auf Wasserbasis, das mit Gel Mount™ vergleichbar ist.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Cy is a registered trademark of Cytiva
Cy3 is a trademark of Cytiva
FluoroShield is a trademark of ImmunoBioScience Corp.
Gel Mount is a trademark of Biomeda Corp.
Texas Red is a registered trademark of Life Technologies
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) immunofluorescence: suitable | technique(s) immunofluorescence: suitable | technique(s) immunofluorescence: suitable | technique(s) - |
| form liquid | form liquid | form liquid | form liquid |
| solubility water: soluble | solubility water: soluble | solubility - | solubility - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
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Lagerklasse
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hi, does this contain any quats/surfactants? Thank you
1 answer-
Helpful?
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What is the refractive index of this product (Fluoroshield Histology Mounting Medium, F6182)?
1 answer-
The refractive index is 1.364 ± 0.002. This number applies to this mounting medium in solution. Refractive indexes change when the mounting media dries on the slide.
Helpful?
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Does this product harden as it dries? Also, what is it's shelf life?
1 answer-
This is an aqueous mounting medium and will dry over time if exposed to air, however this is unrelated to the intended use of the product. The mounting medium is applied over the sample prior to the addition of the coverslip. See the brief protocol below:
1. Bring the vial to room temperature.
2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, then touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light.
3. Turn the vial upside down and remove any air bubbles.
4. Apply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel.
5. Let stand at room temperature for about 5 minutes.
6. Apply cover slip, carefully avoiding air bubbles.
7. The specimen is ready for visualization under microscope.
8. One can seal the edges of cover slip with nail polish or any organic medium. If coverslip is not used air bubbles will appear in few days.
9. For long term storage it is recommended that the slide be stored in the dark at 2-8°C.This product is assigned an expiration date of approximately one year. Please view the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/F6182-20ML-PWMKCT0475.pdf.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
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Does Fluoroshield (Product Nos. F6182, F5932, F6057) contain an antifade reagent?
1 answer-
This unique formula prevents rapid photobleaching. The exact nature of the antifade is considered proprietary.
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How do you remove the coverslip when using Fluoroshield (Product Nos. F6182, F5932, F6057)?
1 answer-
The coverslip can be removed before sealing the edges. Soak slide in warm (37 °C) water for a few minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.
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How do I store Fluoroshield (Product Nos. F6182, F5932, F6057) ?
1 answer-
This product is stored at 2-8°C. Protect from light, and do not freeze.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How do I use Fluoroshield (Product Nos. F6182, F5932, F6057)?
1 answer-
1. Bring the vial to room temperature. 2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, then touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light. 3. Turn the vial upside down and remove any air bubbles. 4. Apply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel. 5. Let stand at room temperature for about 5 minutes. 6. Apply cover slip, carefully avoiding air bubbles. 7. The specimen is ready for visualization under microscope. 8. One can seal the edges of cover slip with nail polish or any organic medium. If coverslip is not used air bubbles will appear in few days. 9. For long term storage it is recommended that the slide be stored in the dark at 2-8°C.
Helpful?
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What fluorochromes can be used with Fluoroshield (Product Nos. F6182, F5932, F6057)?
1 answer-
This unique formula prevents rapid photobleaching of FITC, Texas Red®, AMCA, Cy®2, Cy®3, Cy®5, Alexa Fluor® 488, Alexa Fluor® 594, green fluorescent protein (GFP), tetramethylrhodamine, R-phycoerythrin (R-PE), phycocyanin (PC), and allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of the Cy dyes, RP-E, PC and APC.
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