跳轉至內容
Merck
全部照片(1)

文件

S0902

Sigma-Aldrich

SSC缓冲剂

for Northern and Southern blotting, powder blend

同義詞:

柠檬酸钠盐缓冲液

登入查看組織和合約定價
全部照片(1)

About This Item

分類程式碼代碼:
41105319
NACRES:
NA.25

形狀

powder blend

pH值

6.8-7.2 (20-25 °C, 263 mg/L in water)

溶解度

water: 263 mg/mL, clear, colorless

異物活動

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

儲存溫度

room temp

相關類別

一般說明

SSC缓冲液20x粉末是Southern和Northern blotting的一种标准试剂。该浓缩后的粉末应在使用前用分子生物学级水重构至指定的最终体积（终溶液为20x）。该浓缩液可直接使用，或稀释后制成浓度低至0.5x的SSC工作溶液。

應用

柠檬酸钠盐（SSC）缓冲液在微阵列技术中已被用作洗涤液。它也被用作荧光原位杂交（FISH）缓冲液中的一种组分。
适用于：
  • 制备用于杂交的核酸
  • 制备用于Northern和Southern印迹的杂交缓冲液
  • 制备用于Northern和Southern印迹的洗涤液

特點和優勢

  • 在杂交中具有多种用途
  • 可简单的被重构并稀释成任何所需的浓度

成分

20x的SSC缓冲液终浓缩液在3M NaCl中含有0.3 M柠檬酸钠。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
Bolanos V, et al.
The Plant Journal (2013)
AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects
Papp D, et al.
Autophagy, 12(2), 273-286 (2016)
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how

條款

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern 和 Southern 印迹协议

學習 Northern 和 Southern 印迹的基本知識，以及將大分子轉移到膜支持物上的協議和應用。

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務