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Merck
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T9285

Sigma-Aldrich

Tris-EDTA 缓冲液

100X concentrate for Northern and Southern blotting

同義詞:

Tris-EDTA洗涤液, TE 缓冲液 溶液

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About This Item

MDL號碼:
分類程式碼代碼:
41105319
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology

品質等級

無菌

0.2 μm filtered

形狀

liquid

pH值

7.8-8.2 (1 × in water)

應用

sample preparation

異物活動

DNase, RNase, none detected

儲存溫度

room temp

SMILES 字串

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI 密鑰

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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一般說明

Tris EDTA缓冲液在分子生物学中常用于很多生物分子沉淀物(包括DNA和RNA)的重悬和保存。其中,Tris-HCl是常用缓冲液,EDTA是金属螯合剂。TE缓冲液可溶解核酸,同时保护其免受降解。可用于核酸分离,比如核酸凝胶电泳、Northern或Southern印迹杂交前的准备。

應用

TE缓冲液已用于:
  • 分离、重悬或保存DNA和RNA用于PCR或qPCR
  • 凝胶电泳,包括Northern和Southern免疫印迹分析
  • 细菌细胞,用于通过AmpC纸片试验检测细胞释放的β-内酰胺酶
  • 免疫组化的抗原修复

成分

TE缓冲液包含1M Tris-HCl(pH约8.0)和0.1M EDTA。

其他說明

如需了解生化试剂系列的更多信息,请填写此表

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Jennifer A Black et al.
Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are
Sarah Bøje et al.
Immunology and cell biology, 94(2), 185-195 (2015-08-14)
Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel
Virtual Fluorescence Color Channels by Selective Photobleaching in Digital PCR Applied to the Quantification of KRAS Point Mutations
Schlenker F et al.
Analytical Chemistry, 93, 10538-10545 (2021)
Shiraz Tyebji et al.
Cell reports, 31(4), 107573-107573 (2020-04-30)
Germline epigenetic factors influence transgenerational inheritance of behavioral traits upon changes in experience and environment. Immune activation due to infection can also modulate brain function, but whether this experience can be passed down to offspring remains unknown. Here, we show
AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)

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