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重要文件

B6429

Sigma-Aldrich

酪蛋白封闭缓冲液 10x

for Northern and Southern blotting, solution

同義詞:

10x Blocking Buffer

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.54
暫時無法取得訂價和供貨情況

等級

for molecular biology

品質等級

形狀

solution

技術

Southern blotting: suitable

儲存溫度

room temp

一般說明

酪蛋白是存在于乳汁中的磷蛋白。它分为四种亚型,包括αS1-、αS2-、β-和 κ-酪蛋白。[1]
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂,常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。

應用

酪蛋白封闭缓冲液10X已用于:

特點和優勢

  • 封闭性能优于奶粉或 BSA
  • 易于使用的浓缩液
  • 与中性和正电荷膜兼容
  • 与化学发光和比色检测试剂盒兼容

準備報告

使用前用分子生物学级水稀释 10x 封闭缓冲液。

象形圖

Exclamation markEnvironment

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Chadwick M Hales et al.
Proteomics, 16(23), 3042-3053 (2016-10-09)
Despite a key role of amyloid-beta (Aβ) in Alzheimer's disease (AD), mechanisms that link Aβ plaques to tau neurofibrillary tangles and cognitive decline still remain poorly understood. The purpose of this study was to quantify proteins in the sarkosyl-insoluble brain
Anna Ah-San Tang et al.
Science signaling, 10(505) (2017-11-16)
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding
Michelle M Adams et al.
Experimental neurology, 211(1), 141-149 (2008-03-18)
Caloric restriction (CR) is a daily reduction of total caloric intake without a decrease in micronutrients or disproportionate reduction of any one dietary component. CR can increase lifespan reliably in a wide range of species and appears to counteract some
Tanja Kalic et al.
The journal of allergy and clinical immunology. In practice, 8(9), 3084-3092 (2020-05-12)
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
Mihai D Niculescu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes

條款

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Southern 和 Western 印迹中的酪蛋白阻断缓冲液协议包括优化信号的故障排除。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

學習 Northern 和 Southern 印迹的基本知識,以及將大分子轉移到膜支持物上的協議和應用。

相關內容

Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.

Questions

1–5 of 5 Questions  
  1. whats the percentage casein in 10X of B6429-500ml ?

    1 answer
    1. Unfortunately, the concentration of casein is considered proprietary.

      Helpful?

  2. Will my RNA samples and probes be safe with this buffer if we are using horseradish peroxidase and chemiluminescent detection?

    1 answer
    1. The 10X Casein Blocking Buffer is recommended for both colorimetric and chemiluminescent detection. It is used as a blocking solution for detection, which occurs after hybridization and is suitable for Northern and Southern applications.

      Helpful?

  3. What is the pH? What is the counter ion? Is there saline salt in the preparation (0.15M)?

    1 answer
    1. Unfortunately, the composition of this product is proprietary and can't be disclosed.

      Helpful?

  4. What is the % w/v casein in this solution?

    1 answer
    1. The casein concentration is 1.8% of the total solution.

      Helpful?

  5. What is the solution solvent? What grade of reagent is this in terms of sterility and associated testing?

    1 answer
    1. As mentioned in the product detail page, this product is a molecular biology-grade product. A solution is prepared by diluting the 10x Blocking Buffer with molecular biology-grade water.

      Helpful?

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