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Merck
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D9905

Sigma-Aldrich

Denhardt′s溶液

for Northern and Southern blotting, lyophilized powder

同義詞:

用于印迹技术的Denhardt′s 溶液

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

等級

for molecular biology

形狀

lyophilized powder

技術

Southern blotting: suitable

異物活動

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

儲存溫度

2-8°C

相關類別

一般說明

Denhardt溶液是一种阻断剂，通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液，可稀释至任何浓度，用于各种预杂交和杂交缓冲液。

應用

Denhardt溶液已被用于：
  • 作为杂交缓冲液的组分，用于脑切片的杂交
  • 用于原位杂交过氧化物酶-（抗过氧化物酶法-全标本包埋）（ISHPAP-WM）过程
  • 用于杂交混合物的制备

适合用于Southern和Northern印迹膜的封闭缓冲液。

生化／生理作用

Denhardt溶液和明胶-铬明矾可用于处理染色体，从而在原位杂交后用胰蛋白酶处理以实现染色体的g显带。

成分

每管Denhardt粉末含有50mg牛血清白蛋白（BSA）、50mg Ficoll和50mg聚乙烯吡咯烷酮（PVP）。

準備報告

制备50倍浓缩液，将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
M Simpson-Holley et al.
Journal of virology, 85(4), 1581-1593 (2010-12-15)
Vaccinia virus (VV) mutants lacking the double-stranded RNA (dsRNA)-binding E3L protein (ΔE3L mutant VV) show restricted replication in most cell types, as dsRNA produced by VV activates protein kinase R (PKR), leading to eIF2α phosphorylation and impaired translation initiation. Here
E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.
Alterations in m-RNA expression for Cu, Zn-superoxide dismutase and glutathione peroxidase in the basal ganglia of MPTP-treated marmosets and patients with Parkinson?s disease
Kunikowska G and Jenner P
Brain Research, 968(2), 206-218 (2003)
Kazuya Morikawa et al.
PLoS pathogens, 8(11), e1003003-e1003003 (2012-11-08)
It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
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