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重要文件

93017

Sigma-Aldrich

SSC缓冲液20倍浓缩液

for Northern and Southern blotting, solution

同義詞:

20x SSC

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25
暫時無法取得訂價和供貨情況

等級

Molecular Biology
for molecular biology

產品線

BioReagent

形狀

solution

技術

Southern blotting: suitable

雜質

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH值

7.0±0.2

密度

1.16 g/mL at 20 °C

儲存溫度

room temp

一般說明

SSC缓冲液20倍浓缩液是Southern和Northern印迹法的标准试剂。该浓缩配方可直接使用,或稀释后制成浓度低至0.5倍的SSC工作溶液。

應用

适用于:
  • 制备用于杂交的核酸
  • 制备用于Northern和Southern印迹的杂交缓冲液
  • 制备用于Northern和Southern印迹的洗涤缓冲液

特點和優勢

  • 方便的即用型液体
  • 在杂交中具有多种用途
  • 可轻松稀释至所需浓度

準備報告

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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分析證明 (COA)

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Human pluripotent stem cells (hPSCs) hold great promise for applications in regenerative medicine. However, the safety of cell therapy using differentiated hPSC derivatives must be improved through methods that will permit the transplantation of homogenous populations of a specific cell
Sterghios A Moschos et al.
BMC genomics, 8, 240-240 (2007-07-21)
At present, nothing is known of the role of miRNAs in the immune response in vivo despite the fact that inflammation is thought to underlie multiple acute and chronic diseases. In these circumstances, patients are commonly treated with corticosteroids such

文章

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

條款

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

學習 Northern 和 Southern 印迹的基本知識,以及將大分子轉移到膜支持物上的協議和應用。

Questions

  1. Hi how long time can I keep open sscx20 solution? What the expire time for this? Same question i have for ddulitions for this bbuffer, f.eks 2xssc?

    1 answer
    1. Provided the product is handled proprerly and not contaminated, it is quite stable. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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