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Merck
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文件

F9037

Sigma-Aldrich

甲酰胺

BioReagent, ≥99.5% (GC), for molecular biology

同義詞:

C1 酰胺, 氨基甲醛

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About This Item

線性公式:
HCONH2
CAS號碼:
75-12-7
分子量：:
45.04
Beilstein:
505995
EC號碼:
200-842-0
MDL號碼:
MFCD00007941
分類程式碼代碼:
12352111
PubChem物質ID:
24894985
NACRES:
NA.52

等級

for molecular biology

品質等級

200

蒸汽密度

1.55 (vs air)

蒸汽壓力

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

產品線

BioReagent

化驗

≥99.5% (GC)

形狀

liquid

自燃溫度

932 °F

技術

electrophoresis: suitable
immunohistochemistry: suitable

折射率

n20/D 1.447 (lit.)

pH值

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

密度

1.134 g/mL at 25 °C (lit.)

適合性

suitable for nucleic acid hybridization

儲存溫度

2-8°C

SMILES 字串

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI 密鑰

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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一般說明

甲酰胺是一种衍生自甲酸的透明液体酰胺。甲酰胺可让核酸在室温下变性和复性。

應用

甲酰胺适用于使用标准配方配制的杂交缓冲液。甲酰胺可弱化核酸双链体稳定性，通常在需要较低杂交温度的杂交方案中50％浓度使用。

其他应用包括：

  • 制备用于BrdU免疫组化的小鼠脑切片
  • HeLa细胞FISH分析。
  • 使用变性梯度凝胶电泳法制备用于PCR产物分析的聚丙烯酰胺凝胶（《The Merck Index》第12版）。
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。

特點和優勢

  • 分子生物学应用去离子
  • 封装在氩气填充的棕色瓶中，以防氧化
  • 用于DNA复性或DNA-RNA杂交
  • 可降低双链核酸的热稳定性

適合性

  • 核酸杂交
  • 防冻剂和凝胶稳定剂
  • 弱化核酸双链体稳定性
  • 消除核酸二级结构
  • 制备RNA和DNA应用所需的杂交缓冲液

其他說明

该产品针对分子生物学应用所需去离子化，包装在氩气填充棕色玻璃瓶中防止氧化。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H351,H360D,H373

危險分類

Carc. 2 - Repr. 1B - STOT RE 2 Oral

標靶器官

Blood

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 1

閃點（°F）

305.6 °F

閃點（°C）

152 °C

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

分析證明 (COA)

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T Kaabache et al.
Analytical biochemistry, 232(2), 225-230 (1995-12-10)
The sensitivity of direct solution hybridization of hepatocytes solubilized in guanidium thiocyanate (GuSCN) for detecting alpha 1-acid glycoprotein and albumin mRNAs was studied. The sensitivity of detection was inversely correlated with the DNA concentration. Raising the hybridization temperature from 20
Laurent Geiser et al.
Journal of chromatography. A, 979(1-2), 389-398 (2002-12-25)
A nonaqueous capillary electrophoresis (NACE) method, coupled with either UV or electrospray mass spectrometry (ESI-MS), is described for the simultaneous analysis of seven beta-blockers. The same electrolyte, namely 25 mM ammonium formate and 1 M formic acid, was used with
N C Pagratis
Nucleic acids research, 24(18), 3645-3646 (1996-09-15)
Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C
Jianwei Li
Journal of pharmaceutical sciences, 91(8), 1873-1879 (2002-07-13)
This article describes the proper selection of extraction solvents to eliminate interference from a polymer matrix to the quantitation of estradiol degradation products in a transdermal formulation by reversed-phase liquid chromatography. The separation is performed by gradient elution with acetonitrile
Helena Mistry et al.
Genome integrity, 1(1), 7-7 (2010-08-04)
DNA double-strand breaks (DSBs) caused by ionizing radiation or by the stalling of DNA replication forks are among the most deleterious forms of DNA damage. The ability of cells to recognize and repair DSBs requires post-translational modifications to histones and
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