跳轉至內容
Merck
全部照片(2)

重要文件

P4809

Sigma-Aldrich

磷酸盐-柠檬酸盐缓冲液

tablet

同義詞:

PCR缓冲液

登入查看組織和合約定價


About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25
暫時無法取得訂價和供貨情況

形狀

tablet

品質等級

pH值

4.5-5.5 (1 tablet/100mL in water)

溶解度

water: soluble

儲存溫度

room temp

一般說明

磷酸柠檬酸缓冲液是一种常用的缓冲液,与可溶性辣根过氧化物酶底物一起使用,如邻苯二胺二盐酸(OPD)或四甲基联苯胺(TMB)。它的pH值为5.0,具有出色的缓冲能力。柠檬酸磷酸盐缓冲片可配制0.05 M、pH 5.0的柠檬酸磷酸盐缓冲液。这些片剂提供了一种快速、方便、准确的合成柠檬酸磷酸酯缓冲溶液的方法。它消除了在缓冲液制备中称重单个成分的耗时和繁琐的过程。[1]磷酸盐-柠檬酸缓冲片由最高质量的成分制造,以严格的物理和化学规格,以确保性能和批次间的一致性。pH 5.0的磷酸盐-柠檬酸盐缓冲液片剂已在免疫测定中配制为底物缓冲液。

應用


  • Characterization of nettle leaves (Urtica dioica) as a novel source of protease for clotting dromedary milk by non-destructive methods.:在酶促提取过程中使用磷酸-柠檬酸缓冲液保持植物来源酶的稳定性和活性,说明它在乳制品天然混凝剂开发中的重要作用(Bouazizi et al., 2022)。

  • A rapid, low pH, nutrient stress, assay to determine the bactericidal activity of compounds against non-replicating Mycobacterium tuberculosis.:使用磷酸-柠檬酸缓冲液,研究开发一种低pH营养应激检测方法,评估抗菌剂对结核休眠菌的杀菌疗效,提供重要药物开发工具(Early et al., 2019)。

  • Measurement of Free Iodine in Different Formulations of Povidone-Iodine Eye Drops 5.:用磷酸-柠檬酸缓冲液检测滴眼液中碘的稳定性和释放,说明它确保护眼产品疗效和安全性的重要作用(Prado et al., 2019)。

重構

在25℃下,一片溶于100 mL去离子水中可得到0.05 M磷酸盐-柠檬酸盐缓冲液,pH 5.0。室温储存。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Rose, N., et al.
Manual of Clinical Laboratory Immunology, 106-106 (1986)
In vivo experimental model of orthotopic dental pulp regeneration under the influence of photobiomodulation therapy.
Maria Stella Moreira et al.
Journal of photochemistry and photobiology. B, Biology, 166, 180-186 (2016-12-09)
P R Garrido et al.
Journal of photochemistry and photobiology. B, Biology, 194, 149-157 (2019-04-08)
Photobiomodulation therapy (PBMT) and the cell sheet (CS) technology improve processes relevant to tissue regeneration. The aim of this study was to investigate the effects of different PBMT parameters on the architecture (histology), protein composition (Western blotting and immunohistochemistry) and
Antonino Tuttolomondo et al.
Oncotarget, 8(37), 61415-61424 (2017-10-06)
Anderson-Fabry disease (AFD) is an inborn lysosomal enzymopathy resulting from the deficient or absent activity of the lysosomal exogalactohydrolase, α-galactosidase A. This deficiency, results in the altered metabolism of glycosphingolipids which leads to their accumulation in lysosomes, thus to cellular
A S de Mello et al.
Journal of biomedicine & biotechnology, 2011, 132581-132581 (2011-06-11)
The Epstein-Barr virus (EBV) was used as an agent of B lymphocyte proliferation for subsequent diagnosis of lysosomal storage disease. Due to the constant handling of long-preserved samples in our cell bank, we decided to observe the behavior and then

Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Can this tablet be used for coagulation of. Milk for making cheese

    1 answer
    1. This product is intended primarily for laboratory research purposes unless otherwise stated. This product not to be used for any other purposes, including but not limited to, in vitro diagnostic purposes, in foods, drugs, medical devices or cosmetics for humans or animals or for commercial purposes.

      Helpful?

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務