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Merck
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文件

D0428

Sigma-Aldrich

1 kb DNA分子量标准

for DNA electrophoresis

同義詞:

1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

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About This Item

分類程式碼代碼:
41105335
NACRES:
NA.25

品質等級

100
200

形狀

liquid

適合性

suitable for electrophoresis (DNA)

儲存溫度

−20°C

相關類別

一般說明

Sigma的1kb分子量标准含有11个片段，包含0.5至3 kb的500bp重复序列，3至6 kb的1 kb重复序列，以及6至10 kb的2 kb重复序列。在核酸凝胶电泳中，应在凝胶缓冲液中稀释5μl分子量标准，然后在琼脂糖或聚丙烯酰胺凝胶上单泳道上样。适用于Northern和Southern印迹杂交。所有的DNA标记物都可以用溴化乙锭、SYBR® Green和Nancy-520染色。

應用

1 kb DNA分子量标准品已用作通过凝胶电泳测定双链DNA的分子量和大小的分子量标准。

成分

Sigma的1kb分子量标准品以溶于10mM Tris-HCl(pH 8.0)和1.0 mM EDTA的溶液形式提供。

其他說明

为实现最佳分辨率，建议采用的琼脂糖凝胶浓度为0.75%。

法律資訊

SYBR is a registered trademark of Life Technologies

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Luca Cocolin et al.
Applied and environmental microbiology, 70(3), 1347-1355 (2004-03-10)
In this paper we describe the development of a PCR protocol to specifically detect Brettanomyces bruxellensis and B. anomalus. Primers DB90F and DB394R, targeting the D1-D2 loop of the 26S rRNA gene, were able to produce amplicons only when the
Yu Zhou et al.
Journal of experimental botany, 59(10), 2803-2813 (2008-06-03)
The turnip crinkle virus-based vector TCV-GFP Delta CP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing. TCV-GFP Delta CP, which had been constructed by replacing the coat protein (CP) gene with a green fluorescent
Lidia Fuentes et al.
Journal of inorganic biochemistry, 203, 110875-110875 (2019-11-11)
One mononuclear and another dinuclear Pd(II) complexes bearing a α-N-heterocyclic thiosemicarbazone ligand have been synthesized, fully characterized and studied as biological agents. In both complexes, the palladium center is coordinated to 3,5-diacetyl-1,2,4-triazol bis(N4,N4-dimethylthiosemicarbazone) via three sites (N, N and S).
W S Choe et al.
Biotechnology progress, 17(6), 1107-1113 (2001-12-12)
The direct chemical extraction of recombinant L1 protein (the major capsid protein of human papillomavirus type 16) from the cytoplasm of E. coli HMS174(DE3) has recently been demonstrated at high cell density (to OD(600) = 160) without the use of
Ana I Matesanz et al.
Chembiochem : a European journal of chemical biology, 21(8), 1226-1232 (2019-11-21)
The synthesis and characterization of three new platinum complexes, with 3,5-diacetyl-1,2,4-triazole bis(4-N-isopropylthiosemicarbazone) as a ligand, are reported. The specific conditions under which solvent coordination takes place are reported and the X-ray structure of the complex with one solvent molecule of

文章

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

凝膠電泳標記

用於凝膠電泳的標記有助於確定 DNA、PCR 片段和 RNA 的大小，與常見的核酸染色劑染色效果良好。

為凝膠電泳選擇標記和梯子

透過瓊脂糖或聚丙烯酰胺凝膠上的電泳，選擇適當的標記和梯子進行核酸大小測定。

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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