This product is supplied in a unit size. 1 unit of any DNA = 50 µg.
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一般說明
大肠杆菌B株是k-12后研究最广泛的DNA。大肠杆菌B株和k-12的基因在IS序列元素上存在差异。菌株B具有B岛,表达o特异性侧链LPS。[1]这是未剪接的基因组DNA。
特異性
50% GC;Tm = 90.5 °C
應用
單位定義
一个酶活性单位是指在pH 7.5(1 cm光程)条件下,在1.0 ml的1 mM Tris-HCl溶液(含有1 mM NaCl和1 mM EDTA)中产生1.0的 A260 所需的酶量。
準備報告
通过阴离子交换色谱法纯化,使用含有1 mM氯化钠、1 mM EDTA和1 mM pH 7.5的 Trizma Hcl析出并以大约2单位/L的浓度冷冻干燥。每mg DNA大约20 A260个单位。 %GC是G/C碱基对的百分比。Tm是热变性曲线中点处的温度。
分析報告
在溴化乙锭染色后通过凝胶电泳和可视化分析,具有45kB或更大的单一主条带。
其他說明
溶液中的DNA在室温下放置后将退火,因此,建议将溶液煮沸10分钟,然后在使用前在冰上冷却至少5分钟。
相關產品
產品號碼
描述
訂價
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個:
分析證明 (COA)
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what is the absolute amount of DNA in this product is it 50µg?
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