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Merck
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31149

Sigma-Aldrich

脱氧核糖核酸,低分子量 来源于鲑鱼精子

同義詞:

鲑鱼精子dsDNA, DNA

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
41106310
eCl@ss:
32160414
NACRES:
NA.52

等級

for molecular biology
puriss. p.a.

形狀

lyophilized powder

雜質

≤5% protein

溶解度

50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow

吸光率

A260/280 nm ~1.4

儲存溫度

2-8°C

應用

从鲑鱼精子中提取的不同浓度的低分子量脱氧核糖核酸已用于:
  • 研究DNA与硅取代羟基磷灰石纳米晶体的结合。
  • 在简单的电化学DNA生物传感器中检测和评估UV-C辐射和活性氧造成的DNA损伤。
  • 作为合成生物点的前体。

生化/生理作用

在各种研究应用中,鲑鱼精子DNA常被用作阻断剂。 已知它可以通过减少非特异性结合和背景信号来提高多AP测定的特异性和敏感性。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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David Czeiger et al.
American journal of clinical pathology, 135(2), 264-270 (2011-01-14)
Elevated circulating cell-free DNA (CFD) levels were found in patients with cancer. The standard CFD assays are work-intensive and expensive. The aim was to evaluate in patients with cancer a new simple CFD assay. In mice inoculated with cancer cells
Jing Yu et al.
Molecular biology of the cell, 13(2), 558-569 (2002-02-21)
Dramatic changes occur in nuclear organization and function during the critical developmental transition from meiosis to mitosis. The Drosophila nuclear lamina protein YA binds to chromatin and is uniquely required for this transition. In this study, we dissected YA's binding
J J Gao et al.
Journal of immunology (Baltimore, Md. : 1950), 166(11), 6855-6860 (2001-05-22)
LPS is well recognized for its potent capacity to activate mouse macrophages to produce TNF-alpha, an important inflammatory mediator in bacterial infection-related diseases such as septic shock. We demonstrate here that while inducing only low levels of TNF-alpha alone, DNA
Eiji Sunami et al.
Clinical chemistry, 55(3), 559-567 (2009-01-10)
Prostate cancer (PCa) detection using serum-based prostate specific antigen (PSA) is limited by frequent false-positive and -negative results. Genetic aberrations such as allelic imbalance (AI) and epigenetic changes such as promoter hypermethylation have been detected in circulating DNA of cancer
V N Kondratova et al.
Biochemistry. Biokhimiia, 74(11), 1285-1288 (2009-11-18)
A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters

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