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N6019

Sigma-Aldrich

Neutralizing Buffer

for Northern and Southern blotting, powder

同義詞:

Neutralizing Buffer for Blotting Techniques

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

等級

for molecular biology

形狀

powder

技術

Southern blotting: suitable

異物活動

DNase, RNase, none detected

儲存溫度

room temp

一般說明

Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.

應用

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

成分

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)

條款

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

學習 Northern 和 Southern 印迹的基本知識,以及將大分子轉移到膜支持物上的協議和應用。

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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