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Merck
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C7594

Sigma-Aldrich

酪蛋白封闭缓冲液

for Northern and Southern blotting, powder blend

同義詞:

blocking buffer

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About This Item

EC號碼:
分類程式碼代碼:
12161700
NACRES:
NA.25

等級

for molecular biology

品質等級

形狀

powder blend

技術

Southern blotting: suitable

pH值

6.8-7.2(reconstituted per directions)

溶解度

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

儲存溫度

room temp

一般說明

酪蛋白是一种牛奶来源的物质,通常用于 Western、Southern 和 Northern 印迹实验中的封闭缓冲液。封闭剂用于减少检测试剂与膜的非特异性结合。

應用

适合用作 Western、Southern 和 Northern 印迹膜的封闭缓冲液。然后,封闭膜可用于化学发光,比色或 DIG 检测分析。

特點和優勢

  • 封闭性能优于奶粉或 BSA
  • 与中性和正电荷膜兼容
  • 与化学发光,比色和 DIG 检测分析兼容

準備報告

将瓶中的全部内容物溶解在 800 mL 分子生物学级水中。搅拌直至充分混合,然后加水至终体积 1L。在 2-8°C 下最多保存一周。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1


分析證明 (COA)

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Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Elizabeth R Sunderhaus et al.
Frontiers in neuroscience, 13, 1207-1207 (2019-11-30)
Mutations in patatin-like phospholipase domain-containing protein 6 (PNPLA6) have been linked with a number of inherited diseases with clinical symptoms that include spastic paraplegia, ataxia, and chorioretinal dystrophy. PNPLA6 is an evolutionary conserved protein whose ortholog in Drosophila is Swiss-Cheese

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Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Southern 和 Western 印迹中的酪蛋白阻断缓冲液协议包括优化信号的故障排除。

學習 Northern 和 Southern 印迹的基本知識,以及將大分子轉移到膜支持物上的協議和應用。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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