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P1415

Sigma-Aldrich

预杂交溶液

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

品質等級

形狀

liquid (2x concentrate)

異物活動

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

儲存溫度

−20°C

一般說明

Sigma的预杂交液是一种2倍浓缩的即用型杂交缓冲液,适用于Northern和Southern印迹方案。

應用

适合用作Northern和Southern印迹方案的预杂交缓冲液。

成分

Sigma的2倍预杂交液含200 μg/mL DNA,溶剂为10倍SSC和2倍Denhardt溶液:
  • 200 μg/mL DNA
  • 2mg牛血清白蛋白(BSA)
  • 2mg聚蔗糖
  • 2mg聚乙烯吡咯烷酮(PVP)
  • 0.15 M柠檬酸钠
  • 1.5M NaCl

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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T M Lau et al.
Human reproduction (Oxford, England), 11(12), 2629-2634 (1996-12-01)
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Elizabeth S Colombo et al.
Investigative ophthalmology & visual science, 48(4), 1793-1800 (2007-03-29)
The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Andrew M Pilling et al.
Toxicologic pathology, 35(3), 348-355 (2007-04-25)
Spontaneous follicular and C-cell tumors of the thyroid gland in the Han Wistar rat were examined using two morphologic procedures. Firstly, in situ hybridization (ISH) was used to localize thyroglobulin (TG) and calcitonin (CT) mRNAs. Secondly, the proteins for these
Jianmin Su et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(39), 7421-7435 (2020-08-28)
GABAergic interneurons represent a heterogenous group of cell types in neocortex that can be clustered based on developmental origin, morphology, physiology, and connectivity. Two abundant populations of cortical GABAergic interneurons include the low-threshold, somatostatin (SST)-expressing cells and the fast-spiking, parvalbumin

條款

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

學習 Northern 和 Southern 印迹的基本知識,以及將大分子轉移到膜支持物上的協議和應用。

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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