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Merck

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P1415

预杂交溶液

for Northern and Southern blotting, liquid (2x concentrate)

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NACRES:
NA.25
UNSPSC Code:
12161700
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Quality Segment

form

liquid (2x concentrate)

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

-10 to -25°C

General description

Sigma的预杂交液是一种2倍浓缩的即用型杂交缓冲液,适用于Northern和Southern印迹方案。

Application

适合用作Northern和Southern印迹方案的预杂交缓冲液。

Preparation Note

Sigma的2倍预杂交液含200 μg/mL DNA,溶剂为10倍SSC和2倍Denhardt溶液:
  • 200 μg/mL DNA
  • 2mg牛血清白蛋白(BSA)
  • 2mg Ficoll
  • 2mg聚乙烯吡咯烷酮(PVP)
  • 0.15 M柠檬酸钠
  • 1.5M NaCl

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本產品
H7140H7033S2404
description

for Northern and Southern blotting, liquid (2x concentrate)

description

for Northern and Southern blotting, liquid (2x concentrate)

description

for Northern and Southern blotting, solution

description

pH 7.0±0.05 (25 °C), BioXtra, Molecular Biology, 3 M, non-sterile; 0.2 μm filtered

Quality Level

100, 200

Quality Level

100

Quality Level

200

Quality Level

200

form

liquid (2x concentrate)

form

liquid (2x concentrate)

form

solution

form

liquid

storage temp.

-10 to -25°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

2-8°C

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

-

foreign activity

-


存儲類別/等級

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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