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P4653
Phosphatase, Alkaline from bovine kidney
lyophilized powder, ≥5 units/mg protein (Activity determined in diethanolamine buffer, pH 9.8)
Synonim(y):
Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Polecane produkty
Formularz
lyophilized powder
aktywność właściwa
≥5 units/mg protein (Activity determined in diethanolamine buffer, pH 9.8)
skład
Protein, ~35% biuret
temp. przechowywania
−20°C
Zastosowanie
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins, such as casein, and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P4653 is from bovine kidney and is provided as a lyophilized powder containing Tris-citrate buffer salts.
Działania biochem./fizjol.
Alkaline phosphatase dephosphorylates proteins and nucleic acids.
Definicja jednostki
One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. Previously sold in "glycine" units.
Postać fizyczna
Lyophilized powder containing Tris-citrate buffer salts
Komentarz do analizy
Assays in Tris buffer, pH 10.2 give activity approx. 2X that in the glycine buffer system. Assays in diethanolamine buffer, pH 9.8 give activity approx. 5X that of the glycine buffer system.
Protein determined by biuret
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Inhibitor
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
M Nemir et al.
The Journal of biological chemistry, 264(30), 18202-18208 (1989-10-25)
We have reported previously that the 69-kDa major phosphoprotein, secreted by normal rat kidney (NRK) cells, is osteopontin, a glycosylated bone matrix protein. Here we show that this 69-kDa osteopontin is secreted by NRK cells in both phosphorylated (pp69) and
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To investigate the effects of naringin on the proliferation, differention and maturaion of rat calvarial osteoblasts (ROB). Segregated neonatal SD rat skull, enzyme digestion to obtain ROB. The culture medium was replaced every three days. Serial subcultivation proceeded when cells
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Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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