Aqueous solutions of cysteine oxidize to cystine (and by analogy, N-Acetyl-L-Cysteine to N,N'-Diacetyl-L-Cystine) upon exposure to air at neutral or alkaline pH. The pH of a solution of this product in water has not been measured. So if it is found that the pH is 7 or greater, the addition of a drop or two of dilute hydrochloric acid would be sufficient to stabilize that solution (pH 5, for example). Aqueous stock solutions are stable if frozen for up to 1 month at -20°C.
A9165
N-Acetyl-L-cysteine
≥99% (TLC), suitable for cell culture, BioReagent
N-Acetyl-L-cysteine
Synonim(y):
LNAC, NAC
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Wybierz wielkość
5 G
131,00 zł
25 G
456,00 zł
100 G
1470,00 zł
131,00 zł
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Informacje o tej pozycji
Wzór liniowy:
HSCH2CH(NHCOCH3)CO2H
Numer CAS:
Masa cząsteczkowa:
163.19
NACRES:
NA.26
PubChem Substance ID:
UNSPSC Code:
12352209
EC Number:
210-498-3
MDL number:
Beilstein/REAXYS Number:
1724426
Przejdź do
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
N-Acetyl-L-cysteine, BioReagent, suitable for cell culture
InChI key
PWKSKIMOESPYIA-BYPYZUCNSA-N
InChI
1S/C5H9NO3S/c1-3(7)6-4(2-10)5(8)9/h4,10H,2H2,1H3,(H,6,7)(H,8,9)/t4-/m0/s1
SMILES string
CC(=O)N[C@@H](CS)C(O)=O
product line
BioReagent
assay
≥99% (TLC)
form
powder
technique(s)
cell culture | mammalian: suitable
color
white to off-white
Quality Level
mp
106-108 °C (lit.)
solubility
H2O: 100 mg/mL (with heating)
storage temp.
2-8°C
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Ta pozycja | |||
|---|---|---|---|
| form powder | form powder | form powder | form solid |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) - |
| assay ≥99% (TLC) | assay ≥99% (TLC) | assay ≥99% (TLC) | assay - |
| product line BioReagent | product line - | product line BioXtra | product line - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-30°C |
| mp 106-108 °C (lit.) | mp 106-108 °C (lit.) | mp 106-108 °C (lit.) | mp 110.4 °C |
Application
N-Acetyl-L-cysteine has been used as a component of intestinal basal medium for the culture of mouse intestinal stem cells and also as a component of expansion medium.
Biochem/physiol Actions
Antioxidant and mucolytic agent. Increases cellular pools of free radical scavengers. Reported to prevent apoptosis in neuronal cells but induce apoptosis in smooth muscle cells. Inhibits HIV replication. May serve as a substrate for microsomal glutathione transferase.
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related product
Numer produktu
Opis
Cennik
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Klasa składowania
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Protocol Exchange, 2639-2644 (2017)
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
Kevin P. O?Rourke
Bio-protocol, 6(4), e1733-e1733 (2016)
Meritxell Huch et al.
Nature, 494(7436), 247-250 (2013-01-29)
The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles. A three-dimensional culture system allows long-term clonal expansion of single Lgr5(+) stem
Suzanne M Cloonan et al.
Journal of medicinal chemistry, 58(11), 4494-4505 (2015-05-12)
Ruthenium polypyridyl complexes show great promise as new photodynamic therapy (PDT) agents. However, a lack of detailed understanding of their mode of action in cells poses a challenge to their development. We have designed a new Ru(II) PDT candidate that
Yuval Samuni et al.
Biochimica et biophysica acta, 1830(8), 4117-4129 (2013-04-27)
N-acetylcysteine (NAC) has been in clinical practice for several decades. It has been used as a mucolytic agent and for the treatment of numerous disorders including paracetamol intoxication, doxorubicin cardiotoxicity, ischemia-reperfusion cardiac injury, acute respiratory distress syndrome, bronchitis, chemotherapy-induced toxicity
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is it necessary to adjust the ph if it is prepared in water?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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Is this compound suitable for in vivo i.p. administration (mice)? Or is there an alternate formulation of the N-Acetyl-L-cysteine?
1 answer-
The product is not tested to determine its suitability for in vivo use on laboratory animals, nor is a product available that is known to be suitable for such use.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is this product sterile? If not, can I pass it through a 0.22 um filter unit?
1 answer-
This product is not supplied sterile. However, if the water used is ultrapure, cell culture grade, and prepared in a sterile environment, there is no need to filter the solution.
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Can aliquots be freeze-thawed more than once?
1 answer-
Product A9165 recommended storage is 2-8°C, Regrettably, information about stability after the material is reconstituted is unavailable. Generally speaking, avoid repeated freeze-thawed.
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Can aliquots be stored long term at -80 Celsius?
1 answer-
As mentioned in the previous Q&A, it is recommended to store the aliquoted solutions at -20 °C for up to 1 month. Stability testing at -80°C has not been performed. It is not known if lower storage temperatures will extend the stability of the solution.
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How can I make 1M N-acyteyl cysteine solution? Should I dilute in PBS or distilled water?
1 answer-
This product can be dissolved in H2O up to 100 mg/mL (with heating) . PBS can be used as well. Aliquot and store solutions at -20 deg. C up to 1 month.
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What is the molecular weight of product A9165, N-Acetyl-L-cysteine (NAC)?
1 answer-
As listed on the product detail page, the molecular weight is determined as 163.19
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What concentration of Product A9165, N-Acetyl-L-cysteine (NAC), should I use for cell culture?
1 answer-
Cell culture testing is performed at 0.82 g/L. The exact concentration will depend upon your application.
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