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GE17-0756-01

Glutathione Sepharose™ 4B

Cytiva 17-0756-01, pack of 10 mL

Synonim(y):

affinity chromatography resin, GST-tagged proteins purification,

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About This Item

Kod UNSPSC:
41106500
NACRES:
NA.56

ligand

glutathione

opakowanie

pack of 10 mL

producent / nazwa handlowa

Cytiva 17-0756-01

macierz

4% agarose

wielkość cząstki

45-165 μm

średnia średnica

90 μm

cleaning in place

4-13

working range

4-13

pojemność

>25 mg binding capacity (horse liver GST/ml medium)

Powiązane kategorie

Opis ogólny

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Cechy i korzyści

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informacje prawne

Sepharose is a trademark of Cytiva

Piktogramy

Flame
GHS02

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H226

Zwroty wskazujące środki ostrożności

P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501

Kod klasy składowania

3 - Flammable liquids

Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Xin Zhou et al.
Nature communications, 11(1), 2674-2674 (2020-05-31)
Increasing evidence indicates that guidance molecules used during development for cellular and axonal navigation also play roles in synapse maturation and homeostasis. In C. elegans the netrin receptor UNC-40/DCC controls the growth of dendritic-like muscle cell extensions towards motoneurons and
Amandine Cartier-Michaud et al.
PLoS genetics, 13(6), e1006803-e1006803 (2017-06-16)
Spermatogenesis is a dynamic process that is regulated by adhesive interactions between germ and Sertoli cells. Germ cells express the Junctional Adhesion Molecule-C (JAM-C, encoded by Jam3), which localizes to germ/Sertoli cell contacts. JAM-C is involved in germ cell polarity
Gerard H Pieper et al.
Developmental cell, 53(1), 27-41 (2020-02-29)
Eukaryotic genomes are organized within the nucleus through interactions with inner nuclear membrane (INM) proteins. How chromatin tethering to the INM is controlled in interphase and how this process contributes to subsequent mitotic nuclear envelope (NE) remodeling remains unclear. We have
Ye Guan et al.
eLife, 7 (2018-04-06)
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell
Amandine Draime et al.
Biochimica et biophysica acta, 1861(5), 534-542 (2018-02-23)
Understanding how the activity of transcription factors like HOX proteins is regulated remains a widely open question. In a recent screen for proteins interacting with HOXA1, we identified a PRDM protein family member, PRDM14, which is known to be transiently
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Produkty

Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins

This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Protokoły

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

Moduł wykrywania GST z testem enzymatycznym CDNB

Ta strona opisuje enzymatyczną detekcję białek znakowanych GST za pomocą modułu detekcji GST firmy Cytiva.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

Oczyszczanie przy użyciu Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow i Glutathione Sepharose® 4B

Na tej stronie przedstawiono sposób oczyszczania białek znakowanych GST przy użyciu sefarozy glutationowej firmy Cytiva.

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