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G4510

Millipore

Glutathione−Agarose

lyophilized powder

Synonim(y):

Glutathione-Agarose Resin, GSH-agarose, S-linked glutathione agarose

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About This Item

Numer MDL:
MFCD00131200
Kod UNSPSC:
41106500
NACRES:
NA.56

Postać

lyophilized powder

Poziom jakości

200

klasy chemiczne analitów

proteins (GST)

zakres etykietowania

≥10 μmol per mL gel

metody

immunoprecipitation (IP): suitable
protein purification: suitable

macierz

4% cross-linked beaded agarose

aktywacja macierzy

epoxy

MAR

sulfur

spacer macierzy

12 atoms (10 carbon)

temp. przechowywania

−20°C

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Opis ogólny

Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose.

Zastosowanie

Glutathione-Agarose has been used:
  • to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P
  • to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288)
  • in affinity purification of the fusion protein

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Działania biochem./fizjol.

Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma.

Cechy i korzyści

  • Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes.
  • Has a shelf life of two years if stored properly desiccated at -20 °C.
  • Binding capacity: 5-10 mg glutathione S-transferase per mL resin

Ilość

One g powder swells to ≥10 ml of gel

Postać fizyczna

Lyophilized powder stabilized with lactose
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Sigma-Aldrich

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Powiązane treści

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Techniki ściągania białek

Badanie interakcji białko-białko in vitro za pomocą testów pull-down, z wykorzystaniem metod powinowactwa, GST pull-down, TAP i koimmunoprecypitacji.

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