The [R-H-K-K(Ac)-AFC] substrate is not specific to just the HDAC3. It is recommended to purify the HDAC3 enzyme if necessary.
Przejdź do
EPI004
Histone Deacetylase 3 (HDAC3) Activity Assay Kit
100 assays in 96 well plates
Synonim(y):
Histone deacetylase assay
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Informacje o tej pozycji
usage
100 assays in 96 well plates
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... HDAC3(8841)
mouse ... HDAC3(15183)
General description
Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, it plays a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.
With Sigma′s HDAC3 Activity Assay Kit, HDAC3 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC3 Substrate [R-H-K-K(Ac)-AFC]. This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC3 activity. The kit provides a rapid, simple, sensitive and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC3, and from native, recombinant, or genetically modified HDAC3.
With Sigma′s HDAC3 Activity Assay Kit, HDAC3 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC3 Substrate [R-H-K-K(Ac)-AFC]. This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC3 activity. The kit provides a rapid, simple, sensitive and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC3, and from native, recombinant, or genetically modified HDAC3.
Features and Benefits
- Simple, sensitive, and reliable assay
- Simple procedure; takes ~60 min
- Utilizes fluorometric methods
- Sample type: cell and tissue lysates, plasma and serum, other biological fluids
- Species reactivity: mammalian
- Suitable for individual tests or high throughput assays and kinetic studies
- Convenient 96-well microplate format
- Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples
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Klasa składowania
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - ED ENV 1
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Powiązane treści
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| EPI004-1KT | 04061833609477 |
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I would like to understand if the kit substrate is specific for HDAC3. Assuming that I would use as input a nuclear extract (rich in several nuclear histone deacetylases), would this substrate be catalyzed by all HDACs or doe it only interact with HDAC3?
1 answer-
Helpful?
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