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Dokumenty

A3054

Sigma-Aldrich

Agarose

For pulsed field electrophoresis sample preparation

Synonim(y):

3,6-Anhydro-α-L-galacto-β-D-galactan

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About This Item

Numer CAS:
9012-36-6
Numer WE:
232-731-8
Numer MDL:
MFCD00081294
Kod UNSPSC:
12352201
Identyfikator substancji w PubChem:
24890482
NACRES:
NA.21

pochodzenie biologiczne

algae (red)

Postać

powder

metody

electrophoresis: suitable

zanieczyszczenia

≤10% water

EEO

≤0.10

temp. przejścia

gel point ≤30 °C

ślady anionów

sulfate (SO42-): ≤0.15%

obecność zanieczyszczeń

DNase and RNase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

Klucz InChI

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Zastosowanie

Especially formulated for preparing chromosomal DNA samples prior to pulsed-field gel electrophoresis (PFGE). Making gel plugs for the separation of high molecular weight DNA helps prevent shearing encountered during conventional isolation. Cells can be lysed and the DNA digested with restriction endonucleases while imbedded in agarose plugs.

Komentarz do analizy

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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