T8787

Triton^™ X-100

for molecular biology

• Synonim(y): t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Wzór liniowy: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• Numer CAS: 9036-19-5
• Beilstein: 2315025
• Numer MDL: MFCD00128254
• Kod UNSPSC: 12161900
• Identyfikator substancji w PubChem: 329826885
• NACRES: NA.21

Właściwości

• pochodzenie biologiczne: synthetic (organic)
• Poziom jakości: 200
• klasa czystości: for molecular biology
• opis: non-ionic
• Postać: liquid
• masa cząsteczkowa: micellar avg mol wt 80,000; average mol wt 625
• liczba agregacji: 100-155
• metody: protein purification: suitable; protein quantification: suitable; western blot: suitable
• zanieczyszczenia: <1.00% water (Karl Fischer)
• pH: 9.7
• CMC: 0.2-0.9 mM (20-25°C)
• mp: 6 °C
• temp. przejścia: cloud point 65 °C; pour point ~7 °C
• rozpuszczalność: water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
• gęstość: 1.06 g/mL at 25 °C (lit.)
• ślady kationów: Fe: <5 ppm; K: <0.05%; Na: <0.1%; heavy metals: <5 ppm
• HLB: 13.5
• Zastosowanie: hematology; histology
• obecność zanieczyszczeń: DNase and RNase, none detected
• temp. przechowywania: room temp
• ciąg SMILES: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• Klucz InChI: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Opis

Opis ogólny

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Zastosowanie

Triton^™ X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling

Działania biochem./fizjol.

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Cechy i korzyści

• Ideal for sensitive molecular biology research applications
• Tested for DNase and RNase
• Non-ionic nondenaturing detergent
• Superior wetting agent and emulsifier
• Highly versatile surfactant

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Informacje prawne

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS07,GHS09
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H302,H315,H318,H410
• Zwroty wskazujące środki ostrożności: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): 483.8 °F - closed cup
• Temperatura zapłonu (°C): 251 °C - closed cup
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter