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Merck

D8418

Sigma-Aldrich

Dimethyl sulfoxide

Dimethyl sulfoxide

for molecular biology

Synonim(y):

Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO

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Wybierz wielkość

50 ML
330,00 zł
100 ML
560,00 zł
250 ML
982,00 zł
500 ML
1500,00 zł
1 L
2110,00 zł

330,00 zł


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Wybierz wielkość

Zmień widok
50 ML
330,00 zł
100 ML
560,00 zł
250 ML
982,00 zł
500 ML
1500,00 zł
1 L
2110,00 zł

About This Item

Wzór liniowy:
(CH3)2SO
Numer CAS:
Masa cząsteczkowa:
78.13
Beilstein:
506008
Numer WE:
Numer MDL:
Kod UNSPSC:
12191502
Identyfikator substancji w PubChem:
NACRES:
NA.52
bp:
189 °C (lit.)

330,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

Poproś o zamówienie zbiorcze

klasa czystości

Molecular Biology
for molecular biology

Poziom jakości

gęstość pary

2.7 (vs air)

ciśnienie pary

0.42 mmHg ( 20 °C)

linia produktu

BioReagent

Próba

≥99.9%

Formularz

liquid

temp. samozapłonu

573 °F

okres trwałości

Recommended retest period - 2 years

granice wybuchowości

42 %, 63 °F

metody

DNA sequencing: suitable
PCR: suitable
transfection: suitable

zanieczyszczenia

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

kolor

colorless

współczynnik refrakcji

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

rozpuszczalność

H2O: miscible (completely)

gęstość

1.10 g/mL (lit.)

Absorpcja

passes test

przydatność

suitable for molecular biology

obecność zanieczyszczeń

DNase and RNase, none detected

temp. przechowywania

room temp

ciąg SMILES

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

Klucz InChI

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Opis ogólny

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Zastosowanie

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Cechy i korzyści

This product is free from DNases, RNases, phosphatases and proteases.

Przestroga

Łatwo się schładza i powoli topi w temperaturze pokojowej. Zestalony produkt można ponownie upłynnić poprzez ogrzanie do temperatury pokojowej bez szkody dla produktu.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

188.6 °F - closed cup

Temperatura zapłonu (°C)

87 °C - closed cup

Środki ochrony indywidualnej

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
Claudia C Faria et al.
Cancer research, 75(1), 134-146 (2014-11-14)
Medulloblastoma is the most common malignant pediatric brain tumor, with metastases present at diagnosis conferring a poor prognosis. Mechanisms of dissemination are poorly understood and metastatic lesions are genetically divergent from the matched primary tumor. Effective and less toxic therapies
Mike Clements et al.
Toxicological sciences : an official journal of the Society of Toxicology, 140(2), 445-461 (2014-05-09)
Human stem cell derived cardiomyocytes (hESC-CM) provide a potential model for development of improved assays for pre-clinical predictive drug safety screening. We have used multi-electrode array (MEA) analysis of hESC-CM to generate multi-parameter data to profile drug impact on cardiomyocyte
Björn Zörner et al.
Brain : a journal of neurology, 137(Pt 6), 1716-1732 (2014-04-17)
Anatomical plasticity such as fibre growth and the formation of new connections in the cortex and spinal cord is one known mechanism mediating functional recovery after damage to the central nervous system. Little is known about anatomical plasticity in the

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Questions

1–10 of 18 Questions  
  1. May I know the concentration (mg/ml) for DMSO (cat no. D8418)?

    1 answer
    1. This product is supplied as a neat or pure liquid with a density of 1.10 g/mL and a molecular weight of 78.13 g/mol. The minimum purity is 99.9%, thus the molarity will vary slightly from lot to lot. The molarity is 14.1 M.

      Helpful?

  2. Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?

    1 answer
    1. Yes, Dimethyl sulfoxide (DMSO) is generally considered to be compatible with Polyethylene terephthalate (PET) material. The following information can be found on the Product Data Sheet:

      -Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, teflon FEP
      -Moderately compatible: polystyrene, ECTFE/ETFE
      -Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265/d2650pis.pdf

      Helpful?

  3. Is this product sterile?

    1 answer
    1. This product is not sterile. To prepare a sterile filtered DMSO solution, it is recommended to use a Teflon, polypropylene, or nylon membrane. See products D2650 or D2438 for sterile DMSO product options. Please review these products at the links provided below:
      https://www.sigmaaldrich.com/product/sigma/d2650
      https://www.sigmaaldrich.com/product/sigma/d2438

      Helpful?

  4. DMSO, Product D8418 Dimethyl sulfoxide, is used to freeze and store human and animal cell lines. Is there a procedure for it?

    1 answer
    1. The procedure to freeze cells is in the product information sheet (under Documents, above).

      Helpful?

  5. What can I use to solubilize Product D8418, Dimethyl sulfoxide?

    1 answer
    1. This product is miscible in water (1 mL DMSO + 1 mL H2O), yielding a clear, colorless solution. DMSO is a very hygroscopic liquid and should protected from exposure to moisture. DMSO is also miscible with ethanol, acetone, ether, benzene, and chloroform.

      Helpful?

  6. Why is Product D8418, Dimethyl sulfoxide, considered as an exceptional organic solvent?

    1 answer
    1. Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.

      Helpful?

  7. Is Dimethyl sulfoxide a sterile product?

    1 answer
    1. No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Is Product D8418, Dimethyl sulfoxide, stable under extreme conditions?

    1 answer
    1. DMSO is stable up to 100°C in alkaline, acidic and neutral conditions. At temperatures approaching its boiling point, DMSO is stable in neutral or alkaline conditions.

      Helpful?

  10. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

1–10 of 18 Questions  

Reviews

Active Filters

    • Review 1
    • Votes 2
    5 out of 5 stars.

    DMSO

    it is good for solvent and we use it for long time , it is pure ans stable

    Helpful?

  1. 2 Ratings-Only Reviews

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