This product is supplied as a neat or pure liquid with a density of 1.10 g/mL and a molecular weight of 78.13 g/mol. The minimum purity is 99.9%, thus the molarity will vary slightly from lot to lot. The molarity is 14.1 M.
Kluczowe dokumenty
D8418
Dimethyl sulfoxide
for molecular biology
Synonim(y):
Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
Wybierz wielkość
330,00 zł
Wybierz wielkość
About This Item
330,00 zł
Polecane produkty
klasa czystości
Molecular Biology
for molecular biology
Poziom jakości
gęstość pary
2.7 (vs air)
ciśnienie pary
0.42 mmHg ( 20 °C)
linia produktu
BioReagent
Próba
≥99.9%
Formularz
liquid
temp. samozapłonu
573 °F
okres trwałości
Recommended retest period - 2 years
granice wybuchowości
42 %, 63 °F
metody
DNA sequencing: suitable
PCR: suitable
transfection: suitable
zanieczyszczenia
≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)
kolor
colorless
współczynnik refrakcji
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
rozpuszczalność
H2O: miscible (completely)
gęstość
1.10 g/mL (lit.)
Absorpcja
passes test
przydatność
suitable for molecular biology
obecność zanieczyszczeń
DNase and RNase, none detected
temp. przechowywania
room temp
ciąg SMILES
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
Klucz InChI
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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Opis ogólny
- Suitable for Molecular Applications
- Nuclease-Free DMSO
- DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
- Molecular Biology Grade DMSO
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
Zastosowanie
- polymerase chain reaction (PCR)
- amplification of cDNA libraries
- DNA sequencing
- column-loading buffers for poly(A)+ RNA selection
- buffers for the transformation of competent E. coli
- transfection protocols.
Dimethyl sulfoxide has been used-
- as an oligonucleotide solvent in array spotting[1]
- as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
- for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
- for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
- in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
Cechy i korzyści
Przestroga
najczęściej kupowane z tym produktem
produkt powiązany
zalecane rękawice chroniące przed rozpryskami
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
188.6 °F - closed cup
Temperatura zapłonu (°C)
87 °C - closed cup
Środki ochrony indywidualnej
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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May I know the concentration (mg/ml) for DMSO (cat no. D8418)?
1 answer-
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Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?
1 answer-
Yes, Dimethyl sulfoxide (DMSO) is generally considered to be compatible with Polyethylene terephthalate (PET) material. The following information can be found on the Product Data Sheet:
-Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, teflon FEP
-Moderately compatible: polystyrene, ECTFE/ETFE
-Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonatehttps://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265/d2650pis.pdf
Helpful?
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Is this product sterile?
1 answer-
This product is not sterile. To prepare a sterile filtered DMSO solution, it is recommended to use a Teflon, polypropylene, or nylon membrane. See products D2650 or D2438 for sterile DMSO product options. Please review these products at the links provided below:
https://www.sigmaaldrich.com/product/sigma/d2650
https://www.sigmaaldrich.com/product/sigma/d2438Helpful?
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DMSO, Product D8418 Dimethyl sulfoxide, is used to freeze and store human and animal cell lines. Is there a procedure for it?
1 answer-
The procedure to freeze cells is in the product information sheet (under Documents, above).
Helpful?
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What can I use to solubilize Product D8418, Dimethyl sulfoxide?
1 answer-
This product is miscible in water (1 mL DMSO + 1 mL H2O), yielding a clear, colorless solution. DMSO is a very hygroscopic liquid and should protected from exposure to moisture. DMSO is also miscible with ethanol, acetone, ether, benzene, and chloroform.
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Why is Product D8418, Dimethyl sulfoxide, considered as an exceptional organic solvent?
1 answer-
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
Helpful?
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Is Dimethyl sulfoxide a sterile product?
1 answer-
No, this product is not considered sterile. Product Nos. D2560 and D2438 are sterile-filtered.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product D8418, Dimethyl sulfoxide, stable under extreme conditions?
1 answer-
DMSO is stable up to 100°C in alkaline, acidic and neutral conditions. At temperatures approaching its boiling point, DMSO is stable in neutral or alkaline conditions.
Helpful?
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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