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T7024

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, Molecular Biology, ≥99% (GC)

Synonim(y):

1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

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Do Państwa/SKUDostępnośćCena netto
25 mL
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411,00 zł
50 mL
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705,00 zł
100 mL
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1260,00 zł

Informacje o tej pozycji

Wzór liniowy:
(CH3)2NCH2CH2N(CH3)2
Numer CAS:
Masa cząsteczkowa:
116.20
UNSPSC Code:
41105319
NACRES:
NA.31
PubChem Substance ID:
EC Number:
203-744-6
Beilstein/REAXYS Number:
1732991
MDL number:
Assay:
≥99% (GC)
Grade:
Molecular Biology
Technique(s):
electrophoresis: suitable
Bp:
120-122 °C (lit.)
Vapor pressure:
ca.19.19 hPa

411,00 zł


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grade

Molecular Biology

Quality Segment

vapor pressure

ca.19.19 hPa

product line

BioReagent

assay

≥99% (GC)

expl. lim.

9.08 %

technique(s)

electrophoresis: suitable

refractive index

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

SMILES string

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChI key

KWYHDKDOAIKMQN-UHFFFAOYSA-N

Application

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.[1],,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.[2] TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.[3] Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.[4]
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.[5] TEMED has been used in the HPLC of small organic molecules.[6]

Preparation Note

Suggested use is 35 μl for 50 ml acrylamide gel solution.
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Ta pozycja
T9281411019T22500
description

BioReagent, Molecular Biology, ≥99% (GC)

description

BioReagent, suitable for electrophoresis, ≥99.0%

description

≥99.5%, purified by redistillation

description

ReagentPlus®, 99%

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

assay

≥99% (GC)

assay

≥99.0%

assay

≥99.5%

assay

99%

grade

Molecular Biology

grade

-

grade

-

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

vapor pressure

ca.19.19 hPa

vapor pressure

-

vapor pressure

-

vapor pressure

-

bp

120-122 °C (lit.)

bp

120-122 °C (lit.)

bp

120-122 °C (lit.)

bp

120-122 °C (lit.)


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Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Klasa składowania

3 - Flammable liquids

wgk

WGK 1

flash_point_f

61.7 °F - closed cup

flash_point_c

16.5 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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