Przejdź do zawartości
Merck

D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

Synonim(y):

Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Wzór liniowy:
(CH3)2SO
Numer CAS:
Masa cząsteczkowa:
78.13
Beilstein:
506008
Numer WE:
Numer MDL:
Kod UNSPSC:
12191502
Identyfikator substancji w PubChem:
NACRES:
NA.52

klasa czystości

for molecular biology

Poziom jakości

gęstość pary

2.7 (vs air)

ciśnienie pary

0.42 mmHg ( 20 °C)

linia produktu

BioReagent

Próba

≥99.9%

Postać

liquid

temp. samozapłonu

573 °F

okres trwałości

Recommended retest period - 2 years

granice wybuchowości

42 %, 63 °F

metody

DNA sequencing: suitable
PCR: suitable
transfection: suitable

zanieczyszczenia

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

kolor

colorless

współczynnik refrakcji

n20/D 1.479 (lit.)

tw

189 °C (lit.)

mp

16-19 °C (lit.)

rozpuszczalność

H2O: miscible (completely)

gęstość

1.10 g/mL (lit.)

absorpcja

passes test

przydatność

suitable for molecular biology

obecność zanieczyszczeń

DNase and RNase, none detected

temp. przechowywania

room temp

ciąg SMILES

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

Klucz InChI

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów

Opis ogólny

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Zastosowanie

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Cechy i korzyści

This product is free from DNases, RNases, phosphatases and proteases.

Przestroga

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
This page may contain text that has been machine translated.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

188.6 °F - closed cup

Temperatura zapłonu (°C)

87 °C - closed cup

Środki ochrony indywidualnej

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
Phillip McDonagh et al.
Veterinary microbiology, 174(3-4), 438-447 (2014-12-04)
Feline infectious peritonitis (FIP), a feline coronavirus (FCoV) induced disease, is almost invariably fatal with median life expectancy measured in days. Current treatment options are, at best, palliative. The objectives of this study were to evaluate a panel of nineteen
Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
C F Brayton
The Cornell veterinarian, 76(1), 61-90 (1986-01-01)
Dimethyl sulfoxide (DMSO) is a very simple compound that has stimulated much controversy in the scientific and popular literature. Fig. 1 It is an aprotic solvent. Therapeutic and toxic agents that are not soluble in water are often soluble in
Claudia C Faria et al.
Cancer research, 75(1), 134-146 (2014-11-14)
Medulloblastoma is the most common malignant pediatric brain tumor, with metastases present at diagnosis conferring a poor prognosis. Mechanisms of dissemination are poorly understood and metastatic lesions are genetically divergent from the matched primary tumor. Effective and less toxic therapies

Produkty

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

Protokoły

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

Powiązane treści

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej