10110434001

Oksydaza ksantynowa (XOD)

from cow milk

• Synonim(y): XOD
• Numer EC enzymu: 1.1.3.22 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: bovine milk
• Poziom jakości: 100
• Formularz: suspension
• aktywność właściwa: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
• opakowanie: pkg of 1 mL (20 U)
• producent / nazwa handlowa: Roche
• optymalne pH: 8.5-9.0
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.[1]

Xanthine:oxygen oxidoreductase

Zastosowanie

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP[2][3] and assessment of nitrite-derived NO in liver and purified XOR.[4]

Xanthine Oxidase has been used to study tyrosine nitration.[5]

Działania biochem./fizjol.

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.[6][7]

Jakość

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

Sekwencja

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

Definicja jednostki

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E_293nm = 12.2 mmol ^-1 x L x cm^-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

Postać fizyczna

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

Uwaga dotycząca przygotowania

Activator: O₂
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash