203390

(±)-Blebistatyna

≥97% (HPLC), solid, Myosin II inhibitor, Calbiochem^®

• Synonim(y): (±)-Blebbistatin
• Wzór empiryczny (zapis Hilla): C₁₈H₁₆N₂O₂
• Numer CAS: 674289-55-5
• Masa cząsteczkowa: 292.33
• Numer MDL: MFCD04113775
• Kod UNSPSC: 12352200
• NACRES: NA.77

Właściwości

• Nazwa produktu: (±)-Blebistatyna, (±)-Blebbistatin, CAS 674289-55-5, is a cell-permeable, selective, and reversible inhibitor of nonmuscle myosin II. Blocks cell blebbing and disrupts cell migration & cytoKinesis in vertebrate cells.
• Poziom jakości: 100
• Próba: ≥97% (HPLC)
• Formularz: solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; protect from light
• kolor: yellow
• rozpuszczalność: methanol: 1.5 mg/mL; 100% DMSO: 100 mg/mL; 90% DMSO: 75 mg/mL
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• ciąg SMILES: N2(CCC3(C2=Nc4c(cc(cc4)C)C3=O)O)c1ccccc1
• InChI: 1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3
• Klucz InChI: LZAXPYOBKSJSEX-UHFFFAOYSA-N

Opis

Opis ogólny

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of nonmuscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤ 100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly. A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203389) in 90% DMSO is also available.

Działania biochem./fizjol.

Cell permeable: yes

Primary Target
ATPase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: <100 µM inhibiting the ATPase and gliding motility of human platelets

Opakowanie

Packaged under inert gas

Rekonstytucja

Following reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

Inne uwagi

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable