コンテンツへスキップ
Merck

Collagenase as an effective tool for drug quantitation in tissues.

Bioanalysis (2015-06-04)
Ann Ran-Ran Qin, Xiaorong Liang, Yuzhong Deng, Brian Dean, Sheerin K Shahidi-Latham
要旨

In early drug-discovery research, traditional techniques to analyze drug concentrations in tissues for bioanalytical needs include bead beaters and probe homogenization devices, but are not as effective for tough fibrous tissues. To prepare these tissues, the enzyme collagenase was used to digest the collagen fibers present in epithelial and connective tissue. The benefits of tissue homogenization using a bead beater following collagenase treatment of samples, as opposed to using bead beating alone, was investigated. Matrix effect, recovery factor and stability with and without collagenase were assessed. Little to no effects on the quality and reliability of collagenase treated samples were observed. This enzymatic approach is a feasible and effective tool for tissue homogenization and subsequent analysis by LC-MS/MS.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
ジメチルスルホキシド, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
ジメチルスルホキシド, for molecular biology
Sigma-Aldrich
ジメチルスルホキシド, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
ジメチルスルホキシド, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
塩化カルシウム 溶液, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
コラゲナーゼ Clostridium histolyticum由来, Type IA, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid, For general use
Sigma-Aldrich
塩化カルシウム, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
Sigma-Aldrich
酢酸アンモニウム, for molecular biology, ≥98%
Sigma-Aldrich
酢酸アンモニウム 溶液, for molecular biology, 7.5 M
Sigma-Aldrich
酸化ジルコニウム(IV), powder, 5 μm, 99% trace metals basis
Sigma-Aldrich
ジメチルスルホキシド, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
塩化カルシウム, anhydrous, powder, 99.99% trace metals basis
Sigma-Aldrich
ジメチルスルホキシド, PCR Reagent
Sigma-Aldrich
酸化ジルコニウム(IV), nanopowder, <100 nm particle size (TEM)
Sigma-Aldrich
酢酸アンモニウム, 99.999% trace metals basis
Sigma-Aldrich
アセトニトリル, anhydrous, 99.8%
Sigma-Aldrich
アテノロール, ≥98% (TLC), powder
Sigma-Aldrich
ギ酸, ≥95%, FCC, FG
Sigma-Aldrich
酢酸アンモニウム, JIS special grade, ≥97.0%
Sigma-Aldrich
塩化カルシウム, AnhydroBeads, −10 mesh, ≥99.9% trace metals basis
Sigma-Aldrich
ジメチルスルホキシド, anhydrous, ≥99.9%
Sigma-Aldrich
クロザピン
Sigma-Aldrich
アセトニトリル, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
ギ酸, JIS special grade, ≥98.0%
Sigma-Aldrich
アセトニトリル, ≥99.8%, suitable for HPLC
Sigma-Aldrich
酸化ジルコニウム(IV), 99.99% trace metals basis (purity excludes ~2% HfO2)
Sigma-Aldrich
Calcium chloride
Sigma-Aldrich
アセトニトリル, ≥99.8%, for residue analysis, JIS 300
Sigma-Aldrich
Zirconium(IV) oxide, nanoparticles, dispersion, <100 nm particle size (BET), 10 wt. % in H2O
Sigma-Aldrich
Zirconium(IV) oxide, nanoparticles, dispersion, <100 nm particle size (BET), 5 wt. % in H2O