コンテンツへスキップ
Merck
すべての画像(1)

主要文書

すべての文書を表示

安全性情報

MAK307

Sigma-Aldrich

Malachite Green Phosphate Assay Kit

sufficient for 2500 colorimetric tests

別名:

Phosphate Determination Assay Kit

ログイン組織・契約価格を表示する

サイズを選択してください

1 KIT
￥48,700

￥48,700

出荷可能日2025年4月14日詳細

バルクの問い合わせ
すべての画像(1)

サイズを選択してください

表示を変更する
1 KIT
￥48,700

About This Item

UNSPSCコード:
12161503
NACRES:
NA.84

￥48,700

出荷可能日2025年4月14日詳細

バルクの問い合わせ

使用法

sufficient for 2,500 colorimetric tests

検出方法

colorimetric

関連疾患

cardiovascular diseases; gastrointestinal diseases

保管温度

2-8°C

関連するカテゴリー

詳細

Phosphate is one of the most abundant minerals in the human body and contains about 1 % of total body weight. Phosphate homeostasis is maintained by the several regulators such as parathyroid hormone (PTH), calcitriol and phosphatonins. Maintenance of phosphate homeostasis is essential for various biological processes, such as energy metabolism, cell signaling, regulation of protein synthesis, skeletal development and bone integrity.† An adequate level of phosphate is needed for the process of apoptosis of mature chondrocytes in the growth plate. Phosphate deficiency is associated with the bone pathology and clinical illness.†

アプリケーション

Malachite Green Phosphate Assay Kit has been used in phosphate quantification[1] and steady-state kinetics.[2]
Malachite Green Phosphate Assay Kit has been used to measure the liberation of free orthophosphate in the following applications:
  • Phosphatase Assays: liberation of phosphate from peptide, protein, or small molecule substrate.[1][3]
  • Lipase Assays: liberation of phosphate from phospholipids
  • Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
  • Quantitation of phosphate in phospholipids, proteins and DNAs, etc.
  • Drug Discovery: high-throughput screen for phosphatase inhibitors.

特徴および利点

Compatible with high-throughput handling systems.

適合性

Suitable for phosphate determination in a variety of biological and environmental samples.

原理

Malachite Green is a basic dye belonging to the triphenylmethane group. It is used in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate.†† The Malachite Green Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be executed in tubes, cuvettes or multi-well plates. The assays can be conveniently performed in 96 and 384 well plates for high throughput screening of enzyme inhibitors.

ピクトグラム

FlameCorrosion
GHS02,GHS05

シグナルワード

Warning

危険有害性情報

H226,H290,H315,H319

危険有害性の分類

Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2

保管分類コード

3 - Flammable liquids

引火点(°F)

82.4 °F

引火点(℃)

28 °C

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

毒物及び劇物取締法

キットコンポーネントの情報を参照してください

PRTR

キットコンポーネントの情報を参照してください

消防法

キットコンポーネントの情報を参照してください

労働安全衛生法名称等を表示すべき危険物及び有害物

キットコンポーネントの情報を参照してください

労働安全衛生法名称等を通知すべき危険物及び有害物

キットコンポーネントの情報を参照してください

カルタヘナ法

キットコンポーネントの情報を参照してください

Jan Code

キットコンポーネントの情報を参照してください

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
307CF02A06
307CE08A20
307CE08A13
307CE03A11
307CD12A06

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Ilke Sen et al.
Nature communications, 11(1), 138-138 (2020-01-11)
In C. elegans, the conserved transcription factor DAF-16/FOXO is a powerful aging regulator, relaying dire conditions into expression of stress resistance and longevity promoting genes. For some of these functions, including low insulin/IGF signaling (IIS), DAF-16 depends on the protein SMK-1/SMEK
Julie Wilson et al.
iScience, 23(6), 101219-101219 (2020-06-17)
In macromolecular crystallization, success is often dependent on the pH of the experiment. However, little is known about the pH of reagents used, and it is generally assumed that the pH of the experiment will closely match that of any
Methods in Plant Cell Biology, 50 (1995)
Pho4 Is Essential for Dissemination of Cryptococcus neoformans to the Host Brain by Promoting Phosphate Uptake and Growth at Alkaline pH.
Lev S, et al.
mSphere, 2(1), e00381-e00316 (2017)
Edward G.
Synthetic Dyes in Biology, Medicine And Chemistry (2012)
関連する文献をすべて表示

質問

1–9/9 質問  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 回答

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      役に立ちましたか？

  2. Is this kit able to detect pyrophosphate too?

    1 回答

    1. This kit is intended for the detection of free orthophosphate and has not been tested for pyrophosphate detection. There are other products available that are specifically for use in pyrophosphate detection. Please see the links below to review each item:
      MAK168, Pyrophosphate Assay Kit
      https://www.sigmaaldrich.com/product/sigma/mak168
      MAK169. High Sensitivity Pyrophosphate Assay Kit
      https://www.sigmaaldrich.com/product/sigma/mak169

      役に立ちましたか？

  3. Does the product MAK307 contain magnesium?

    1 回答

    1. Magnesium is not included as a component in any of the reagents provided with the kit for product MAK307. If magnesium serves as an essential cofactor for the enzyme, it is recommended to supplement it in the buffer before conducting the phosphate determination assay.

      役に立ちましたか？

  4. What are the reasons behind the suggestion to use lower concentrations than 0.25mM? Are there potential unwanted biochemical reactions between GTP and malachite green/molybdate if GTP concentration exceeds 0.25mM?

    1 回答

    1. The kit operates by releasing free orthophosphate from ATP or GTP, followed by the creation of a green complex involving the orthophosphate, molybdate, and Malachite green. It has been observed that samples containing ATP and GTP at concentrations exceeding 0.25mM result in the measurement of free phosphate levels falling outside the detection range of the kit (0.02 µM and 40 µM phosphate), and hence should be avoided.

      役に立ちましたか？

  5. Why is it necessary to dilute the ATP concentration to <0.25 mM before the assay? Does ATP interfere with the assay?

    1 回答

    1. BioAssay Support recommends reducing the ATP concentration before the assay to minimize background interference resulting from non-enzymatic ATP hydrolysis and to decrease the likelihood of reagent precipitation with unhydrolyzed ATP.

      役に立ちましたか？

  6. our kit expired, when I used to measure extracted polyphosphate degraded by the enzyme, the results were so different compared to the precious ones (when the kit was not expired), this means the result was not reliable after it expired?

    1 回答

    1. Correct, products used beyond the expiration date will likely offer poor results.

      役に立ちましたか？

  7. Does Malachite Green interact with glycerol?

    1 回答

    1. The Malachite Green Phosphate Assay Kit is based on the quantification of the green complex formed between Malachite Green, molybdate, and free orthophosphate. No specific compatibility study has been done with this kit and glycerol. This would need to be tested empirically, in a sample with a known phosphate concentration, with and without glycerol at the desired concentration.

      役に立ちましたか？

  8. Can we test the same MG in shrimp matrix ?

    1 回答

    1. This product is suitable for a variety of sample types. There is no reason to suspect a problem with shrimp matrix. Attention should be given to sample preparation, handling, and dilution. See the link below to review the product datasheet which includes the protocol and troubleshooting tips:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/171/212/mak307bul.pdf

      役に立ちましたか？

  9. How is shipping temperature determined? And how is it related to the product storage temperature?

    0 回答

レビュー

評価値なし

アクティブなフィルタ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）