The protocol below has not been tested but is based on adaptations of the reagents to similar instruments.
In order to perform the assay in a microplate instead of cuvettes, the reagent and sample volumes can be reduced by a factor of 5. Thus, the reagent volume would be 200 microliters; the volume of water or sample would be 20 microliters, and the volume of standard would be 10 microliters. The required volume of the enzyme solution would decrease to 2 microliters, which is difficult to pipette accurately. To deliver the enzyme more accurately, dilute it 5x P5244 using Phosphate Buffer, pH 7.5, as the enzyme diluent and pipette 10 microliters into each well.
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使用法
sufficient for 100 assays
保管温度
2-8°C
アプリケーション
このキットは、食品や生物学的サンプル中のアンモニアの酵素的定量に用いられます。アンモニアは、L-グルタミン酸脱水素酵素(GlDH)の存在下でα-ケトグルタル酸(KGA)と反応してニコチンアミドアデニンジヌクレオチドリン酸(NADPH)を減少させ、L-グルタミン酸と酸化型ニコチンアミドアデニンジヌクレオチドリン酸(NADP+)を形成します。340 nm吸光度の減少は、NADPHの酸化に起因するもので、アンモニア濃度に比例します。L-グルタミン酸脱水素酵素はアンモニアと特異的に反応します。Ammonia Assay Kitは、0.2～15 μg/mLの範囲のアンモニア濃度の測定に使用可能です。
慢性代謝性アシドーシスに応答した腎臓アンモニア輸送体の発現を評価する研究において、血清および尿中のアンモニア濃度がシグマ社製アンモニアアッセイキットを用いて測定されました。 [1]
キットの構成要素のみ
製品番号
詳細
- Ammonia Assay Reagent 10 vial(s)
- L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial
- Ammonia Standard Solution 1 mL/vial
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Resp. Sens. 1
保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
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PRTR
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消防法
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カルタヘナ法
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Jan Code
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最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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Junpeng Jiang et al.
BMC plant biology, 19(1), 239-239 (2019-06-07)
Ammonium transporters (AMTs), a family of proteins transporting ammonium salt and its analogues, have been studied in many aspects. Although numerous studies have found that ammonium affects the interaction between plants and pathogens, the role of AMTs remains largely unknown
Tingting Xiang et al.
Nature communications, 11(1), 108-108 (2020-01-09)
In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a
Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis.
Seshadri, R.
American Journal of Physiology: Renal Physiology, 290, F397-F408 (2006)
Kahina Mellahi et al.
Bioprocess and biosystems engineering, 42(5), 711-725 (2019-01-24)
Mammalian-inducible expression systems are increasingly available and offer an attractive platform for the production of recombinant proteins. In this work, we have conducted process development for a cumate-inducible GS-CHO cell-line-expressing rituximab. To cope with the limitations encountered in batch when
Na Gao et al.
Fish & shellfish immunology, 65, 226-234 (2017-04-22)
Ammonia is highly toxic to aquatic organisms, but whether ammonia excretion or ammonia metabolism to less toxic compounds is the major strategy for detoxification in marine fish against chronic ammonia exposure is unclear to date. In this study, we investigated
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Can we use this kit for microplate reader system
1 回答-
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf役に立ちましたか？
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In the case of bacterial culture supernatant (BHI broth), should the decolorization process be performed? How?
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Any sample solution that is strongly colored and/or has a low ammonia concentration should be decolorized when using this kit. Colored sample solutions often are treated with decolorizing carbon (activated charcoal) to remove color-contributing impurities.
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Is there a control that can be used to assess the accuracy of Product AA0100, Ammonia Assay Kit?
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A convenient control for use with this kit is the Ammonia Standard Solution that is provided in the kit.
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How can I assess the absorbance change of my reagent blank when using Product AA0100, Ammonia Assay Kit?
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Generally, an absorbance change of 0.050 or less indicates an acceptable range for the reagent blank. An absorbance change higher than this indicates possible contamination of the reagent (or glassware) with ammonia.
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Which samples should be deproteinized for use with Product AA0100, Ammonia Assay Kit?
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The basis for this kit was a Diagnostic procedure for the determination of Ammonia in human plasma, whose total protein concentration is approximately 63 to 83 mg/mL. Thus, any solution whose protein concentration is less than approximately 83 mg/mL should not require deproteinization.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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