MAK461

pNPP Phosphatase Assay Kit

Sufficient for 500 colorimetric tests

• 別名: Phosphatase Assay Kit, p-Nitrophenyl Phosphate Assay Kit
• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 品質水準: 100
• 入力: protein
• 検出方法: colorimetric
• 関連疾患: cancer; hematological disorder; gastrointestinal diseases; neurological disorders; cardiovascular diseases
• 保管温度: −20°C

詳細

詳細

Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A₄₀₅ nm on a spectrophotometer.

アプリケーション

The pNPP Phosphatase Assay Kit may be used for:

• Metabolism Research
• Cardiovascular Research
• Neurodegenerative Research

特徴および利点

Simplified Process: Enjoy a hassle-free procedure that can be completed within 30 minutes without wash or reagent transfer steps, saving you time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

適合性

The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.

原理

The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 °C.

その他情報

For additional information on our range of Biochemicals, please complete this form.

安全性情報

• ピクトグラム: GHS08,GHS05,GHS07,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302 + H332,H314,H341,H373,H411
• 注意書き: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• ターゲットの組織: Nervous system,Kidney,Liver,Skin
• 保管分類コード: 8A - Combustible corrosive hazardous materials