LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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グレード
Molecular Biology
品質水準
無菌性
sterile; autoclaved
フォーム
plates (10 cm)
plates (10cm, 20-25ml each)
キャパシティ
20-25 mL
保管温度
2-8°C
適合性
nonselective for Escherichia coli
nonselective for coliforms
詳細
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of Escherichia coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
アプリケーション
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
LB Agar Plates has been used:
LB Agar Plates has been used:
- to culture bacteria like, Escherichia coli strain INV- α and methicillin-susceptible Staphylococcus aureus[1]
- to culture numerous bacteria and is used in strain storage method[2]
- to analyse the egestion of midgut microbes during sand fly bites[3]
特徴および利点
Lennox LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation
構成
15g/L Agar
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
関連製品
製品番号
詳細
価格
保管分類コード
13 - Non Combustible Solids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
L5542-VAR:
L5542-1EA:
L5542-10EA:
L5542-10EA-PW:
L5542-BULK:
Bacteria Detection and Differentiation Using Impedance Flow Cytometry
Clausen C, et al.
Sensors, 18(10), 3496-3496 (2018)
Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ
Kuru E, et al.
Nature Protocols, 10(1), 33-33 (2015)
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Gut microbes egested during bites of infected sand flies augment severity of leishmaniasis via inflammasome-derived IL-1beta
Dey R, et al.
Cell host & microbe, 23(1), 134-143 (2018)
Alex Golberg et al.
AAPS PharmSciTech, 10(3), 881-886 (2009-07-03)
The purpose of this study was to examine the feasibility of using irreversible electroporation (IRE) as a non-chemical method for eliminating microorganisms of liquid drugs. The studied drug was a topical ophthalmic medication, a pharmaceutical field in which the problem
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 回答-
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Will adding magnesium to the culture medium increase cell density?
1 回答-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the difference among the many LB - Lennox products?
1 回答-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Which bacterial culture medium is the best choice for my application?
1 回答-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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