Leupeptin gives multiple peaks on HPLC because of the formation of tautomeric isomers in solution. Majority of contaminating peptide is racemized leupeptin.
Cell chemical biology, 25(12), 1554-1561 (2018-09-18)
Protein engineering involves generating and screening large numbers of variants for desired properties. While modern DNA technology has made it easy to create protein diversity on the DNA level, the selection and validation of candidate proteins from large libraries remains
Determination of ERK activity: antiphospho-ERK antibodies, in vitro phosphorylation, and in-gel kinase assay.
Sarah Kraus et al.
Methods in molecular biology (Clifton, N.J.), 250, 29-48 (2004-02-03)
Methods in molecular biology (Clifton, N.J.), 832, 403-422 (2012-02-22)
The proteasome is an ATP-dependent molecular machine that degrades proteins through the concerted activity of dozens of subunits. It is the yin to the ribosome's yang, and together these entities mold the protein landscape of the cell. Native gels are
Ionotropic glutamate receptors (iGluRs) are ligand-gated ion channels that play crucial roles in the central nervous system. iGluR homologs, termed glutamate receptor-like channels (GLRs), have been found in plants. Investigating the structural and functional relationship between iGluRs and GLRs was
Molecular biology of the cell, 27(15), 2381-2393 (2016-06-17)
Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making actin
